Reduced surface expression of transforming growth factor β receptor type II in mitogen-activated T cells from Sézary patients

Renold J. Capocasale, Roberta J. Lamb, Eric C. Vonderheid, Floyd E. Fox, Alain H. Rook, Peter C. Nowell, Jonni S. Moore

Research output: Contribution to journalArticlepeer-review

Abstract

Sézary syndrome (SzS), the leukemic form of cutaneous T-cell lymphoma, is characterized by clonal proliferation of CD4+ T cells and immune dysfunctions, raising the possibility of cytokine-related abnormalities. We previously described a decreased response to the growth-inhibitory effects of transforming growth factor type β (TGF-β) in SzS T cells accompanied by apparent loss of surface type IITGF-β receptor (TGFβRII). To specifically determine if defects exist in TGFβRII protein expression and/or transport in SzS patients, we developed a sensitive flow cytometric method to detect TGFβRII on the surface and intracellularly in the CD4+ T cells. Our results indicate that unlike normal CD4+ T cells, CD4+ T cells from 9 of 12 SzS patients expressed little, if any, surface TGFβRII in response to mitogen stimulation. At the intracellular level, however, pools of TGFβRII were comparable to those in normal CD4+ T cells. This indicates that defective trafficking of this inhibitory cytokine receptor may contribute significantly to the development of this disease.

Original languageEnglish (US)
Pages (from-to)5501-5505
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number12
StatePublished - Jun 6 1995
Externally publishedYes

Keywords

  • Cutaneous T-cell lymphoma
  • Endocytosis
  • Flow cytometry
  • Sézary syndrome

ASJC Scopus subject areas

  • General
  • Genetics

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