Reduced expression of the BRCA1 gene and increased chromosomal instability in MCF-7 cell line

S. K. Kachhap; S. P. Vetale; P. Dange; S. N. Ghosh

doi:10.1006/cbir.2000.0657

Reduced expression of the BRCA1 gene and increased chromosomal instability in MCF-7 cell line

S. K. Kachhap, S. P. Vetale, P. Dange, S. N. Ghosh

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Using clonal cell cultures, a significant increase in chromosomal aberrations (aneuplolidy, dicentrics and chromatid breaks) were observed in MCF-7 cells compared with HeLa. BRCA1 expression was lower in MCF-7 cells than in HeLa cells. Since BRCA1 is known to play a role in the maintenance of chromosomal integrity, the increase in chromosomal aberrations in MCF-7 clones suggests that downregulation of BRCA1 expression could be one of the possible mechanisms for increased chromosomal instability in this cell line.

Original language	English (US)
Pages (from-to)	547-551
Number of pages	5
Journal	Cell biology international
Volume	25
Issue number	6
DOIs	https://doi.org/10.1006/cbir.2000.0657
State	Published - 2001
Externally published	Yes

Keywords

BRCA1 mRNA expression
Breast cancer cell line
Chromosomal instability

ASJC Scopus subject areas

Cell Biology

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10.1006/cbir.2000.0657

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title = "Reduced expression of the BRCA1 gene and increased chromosomal instability in MCF-7 cell line",

abstract = "Using clonal cell cultures, a significant increase in chromosomal aberrations (aneuplolidy, dicentrics and chromatid breaks) were observed in MCF-7 cells compared with HeLa. BRCA1 expression was lower in MCF-7 cells than in HeLa cells. Since BRCA1 is known to play a role in the maintenance of chromosomal integrity, the increase in chromosomal aberrations in MCF-7 clones suggests that downregulation of BRCA1 expression could be one of the possible mechanisms for increased chromosomal instability in this cell line.",

keywords = "BRCA1 mRNA expression, Breast cancer cell line, Chromosomal instability",

author = "Kachhap, {S. K.} and Vetale, {S. P.} and P. Dange and Ghosh, {S. N.}",

note = "Funding Information: The authors thank Mrs A. A. Dolas for typing the manuscript. This work was financially supported by Council of Scientific and Industrial Research (CSIR), India, grant No. 37/884/95-EMR-II. The senior research fellowship awarded to S.K.K. by the Indian Council of Scientific and Industrial Research (CSIR) is gratefully acknowledged.",

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doi = "10.1006/cbir.2000.0657",

language = "English (US)",

volume = "25",

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journal = "Cell biology international",

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TY - JOUR

T1 - Reduced expression of the BRCA1 gene and increased chromosomal instability in MCF-7 cell line

AU - Kachhap, S. K.

AU - Vetale, S. P.

AU - Dange, P.

AU - Ghosh, S. N.

N1 - Funding Information: The authors thank Mrs A. A. Dolas for typing the manuscript. This work was financially supported by Council of Scientific and Industrial Research (CSIR), India, grant No. 37/884/95-EMR-II. The senior research fellowship awarded to S.K.K. by the Indian Council of Scientific and Industrial Research (CSIR) is gratefully acknowledged.

PY - 2001

Y1 - 2001

N2 - Using clonal cell cultures, a significant increase in chromosomal aberrations (aneuplolidy, dicentrics and chromatid breaks) were observed in MCF-7 cells compared with HeLa. BRCA1 expression was lower in MCF-7 cells than in HeLa cells. Since BRCA1 is known to play a role in the maintenance of chromosomal integrity, the increase in chromosomal aberrations in MCF-7 clones suggests that downregulation of BRCA1 expression could be one of the possible mechanisms for increased chromosomal instability in this cell line.

AB - Using clonal cell cultures, a significant increase in chromosomal aberrations (aneuplolidy, dicentrics and chromatid breaks) were observed in MCF-7 cells compared with HeLa. BRCA1 expression was lower in MCF-7 cells than in HeLa cells. Since BRCA1 is known to play a role in the maintenance of chromosomal integrity, the increase in chromosomal aberrations in MCF-7 clones suggests that downregulation of BRCA1 expression could be one of the possible mechanisms for increased chromosomal instability in this cell line.

KW - BRCA1 mRNA expression

KW - Breast cancer cell line

KW - Chromosomal instability

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Reduced expression of the BRCA1 gene and increased chromosomal instability in MCF-7 cell line

Abstract

Keywords

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