Abstract
Background: The role of the striatum in the pathophysiology of schizophrenia is not understood. In a previous postmortem study, we found a reduction in the density of striatal interneurons that stain immunohistochemically for choline acetyltransferase (ChAT) in schizophrenia. Methods: To determine whether this finding represents a specific alteration in ChAT gene expression, we used in situ hybridization to study the striatum of 11 control and 9 schizophrenic subjects with oligonucleotide probes complementary to human ChAT mRNA, preprosomatostatin (PPS) mRNA, and β-actin mRNA. Densities of ChAT mRNA-positive neurons, ChAT mRNA expression per neuron, PPS mRNA-positive neurons, and β-actin mRNA expression levels were measured. Results: There were no significant differences between the two groups in densities of PPS mRNA-positive neurons and levels of β-actin mRNA expression throughout the striatum, or in densities of ChAT mRNA-positive neurons in the caudate nucleus or putamen. However, in the ventral striatum, the mean density of ChAT mRNA-positive neurons was reduced to 26% of control levels in the schizophrenic group. Conclusions: There is a reduction in number or function of the cholinergic interneurons of the ventral striatum in schizophrenia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 408-416 |
| Number of pages | 9 |
| Journal | Biological Psychiatry |
| Volume | 58 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 1 2005 |
| Externally published | Yes |
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Keywords
- Basal ganglia
- Cholinergic
- In situ hybridization
- Postmortem
- Schizophrenia
- Striatum
ASJC Scopus subject areas
- Biological Psychiatry
Cite this
Reduced density of cholinergic interneurons in the ventral striatum in Schizophrenia : An in situ hybridization study. / Holt, Daphne J.; Bachus, Susan E.; Hyde, Thomas; Wittie, Michael; Herman, Mary M.; Vangel, Mark; Saper, Clifford B.; Kleinman, Joel.
In: Biological Psychiatry, Vol. 58, No. 5, 01.09.2005, p. 408-416.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduced density of cholinergic interneurons in the ventral striatum in Schizophrenia
T2 - An in situ hybridization study
AU - Holt, Daphne J.
AU - Bachus, Susan E.
AU - Hyde, Thomas
AU - Wittie, Michael
AU - Herman, Mary M.
AU - Vangel, Mark
AU - Saper, Clifford B.
AU - Kleinman, Joel
PY - 2005/9/1
Y1 - 2005/9/1
N2 - Background: The role of the striatum in the pathophysiology of schizophrenia is not understood. In a previous postmortem study, we found a reduction in the density of striatal interneurons that stain immunohistochemically for choline acetyltransferase (ChAT) in schizophrenia. Methods: To determine whether this finding represents a specific alteration in ChAT gene expression, we used in situ hybridization to study the striatum of 11 control and 9 schizophrenic subjects with oligonucleotide probes complementary to human ChAT mRNA, preprosomatostatin (PPS) mRNA, and β-actin mRNA. Densities of ChAT mRNA-positive neurons, ChAT mRNA expression per neuron, PPS mRNA-positive neurons, and β-actin mRNA expression levels were measured. Results: There were no significant differences between the two groups in densities of PPS mRNA-positive neurons and levels of β-actin mRNA expression throughout the striatum, or in densities of ChAT mRNA-positive neurons in the caudate nucleus or putamen. However, in the ventral striatum, the mean density of ChAT mRNA-positive neurons was reduced to 26% of control levels in the schizophrenic group. Conclusions: There is a reduction in number or function of the cholinergic interneurons of the ventral striatum in schizophrenia.
AB - Background: The role of the striatum in the pathophysiology of schizophrenia is not understood. In a previous postmortem study, we found a reduction in the density of striatal interneurons that stain immunohistochemically for choline acetyltransferase (ChAT) in schizophrenia. Methods: To determine whether this finding represents a specific alteration in ChAT gene expression, we used in situ hybridization to study the striatum of 11 control and 9 schizophrenic subjects with oligonucleotide probes complementary to human ChAT mRNA, preprosomatostatin (PPS) mRNA, and β-actin mRNA. Densities of ChAT mRNA-positive neurons, ChAT mRNA expression per neuron, PPS mRNA-positive neurons, and β-actin mRNA expression levels were measured. Results: There were no significant differences between the two groups in densities of PPS mRNA-positive neurons and levels of β-actin mRNA expression throughout the striatum, or in densities of ChAT mRNA-positive neurons in the caudate nucleus or putamen. However, in the ventral striatum, the mean density of ChAT mRNA-positive neurons was reduced to 26% of control levels in the schizophrenic group. Conclusions: There is a reduction in number or function of the cholinergic interneurons of the ventral striatum in schizophrenia.
KW - Basal ganglia
KW - Cholinergic
KW - In situ hybridization
KW - Postmortem
KW - Schizophrenia
KW - Striatum
UR - http://www.scopus.com/inward/record.url?scp=24044528136&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24044528136&partnerID=8YFLogxK
U2 - 10.1016/j.biopsych.2005.04.007
DO - 10.1016/j.biopsych.2005.04.007
M3 - Article
C2 - 16023618
AN - SCOPUS:24044528136
VL - 58
SP - 408
EP - 416
JO - Biological Psychiatry
JF - Biological Psychiatry
SN - 0006-3223
IS - 5
ER -