Redistribution of Na+/H1 exchanger isoform NHE3 in proximal tubules induced by acute and chronic hypertension

Kay Pong Yip, Chung Ming Tse, Alicia A. Mcdonough, Donald J. Marsh

Research output: Contribution to journalArticle

Abstract

Redistribution of apical Na+/H+ exchangers (NHE) in the proximal tubules as a plausible mechanism of pressure natriuresis was investigated with confocal immunofluorescence microscopy in Sprague-Dawley rats (SD), spontaneously hypertensive rats (SHR), and two-kidney, one-clip Goldblatt hypertensive rats (GH). NHE isoform NHE3 was localized in the brush border of proximal tubules in SD. Twenty minutes of induced acute hypertension (20-40 mmHg) resulted in a pronounced redistribution of isoform NHE3 from the brush border into the base of microvilli, where clathrin-coated pits were localized. Prehypertensive young SHR (5 wk old, mean blood pressure 105 ± 3 mmHg, n = 11) produced similar findings. However, NHE3 was found to concentrate in the base of microvilli in adult SHR (12 wk old, mean blood pressure 134 ± 6 mmHg, n = 12) and nonclipped kidneys of GH (mean blood pressure 131 ± 6 mmHg, n = 6). In clipped kidneys of GH, which were not exposed to the hypertension because of the arterial clips, NHE3 was localized on the brush border as in normal SD. No further redistribution of NHE3 was detected in adult SHR or GH when acute hypertension was induced. Since both acute and chronic increase of arterial pressure can provoke the redistribution of apical NHE in proximal tubules, the pressure-induced NHE redistribution could be a physiological response and an integral part of pressure natriuresis.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume275
Issue number4 44-4
StatePublished - Oct 1998

Fingerprint

Microvilli
Sodium-Hydrogen Antiporter
Inbred SHR Rats
Protein Isoforms
Hypertension
Sprague Dawley Rats
Natriuresis
Blood Pressure
Kidney
Surgical Instruments
Pressure
Clathrin
Fluorescence Microscopy
Confocal Microscopy
Arterial Pressure

Keywords

  • Confocal fluorescence microscopy
  • Genetic hypertension
  • Goldblatt hypertension
  • Immunofluorescence microscopy
  • Pressure natriuresis

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Redistribution of Na+/H1 exchanger isoform NHE3 in proximal tubules induced by acute and chronic hypertension. / Yip, Kay Pong; Tse, Chung Ming; Mcdonough, Alicia A.; Marsh, Donald J.

In: American Journal of Physiology - Renal Physiology, Vol. 275, No. 4 44-4, 10.1998.

Research output: Contribution to journalArticle

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