TY - JOUR
T1 - Redistribution of membrane proteins in isolated mouse intestinal epithelial cells
AU - Ziomek, Carol Ann
AU - Schulman, Seth
AU - Edidin, Michael
PY - 1980/9/1
Y1 - 1980/9/1
N2 - Single mouse intestinal epithelial cells (IEC) may be isolated by the use of a combination of methods used for the isolation of IEC from other species. Isolated cells remain viable for several hours. The membrane integral enzymes alkaline phosphatase and leucine aminopeptidase of isolated IEC are localized to the brush borders of IEC in tissue and in most newly isolated IEC. With time, both enzymes are found distributed over the entire cell surface. Redistribution appears to occur by diffusion in the plane of the membrane. It is slowed, but not blocked, if cells are maintained at 0° C instead of at 37° C, and it is not blocked by fixation in 0.5-3% paraformaldehyde. Drugs that alter cell membrane potential or that affect cell levels of ATP enhance the rate of redistribution of the enzymes.
AB - Single mouse intestinal epithelial cells (IEC) may be isolated by the use of a combination of methods used for the isolation of IEC from other species. Isolated cells remain viable for several hours. The membrane integral enzymes alkaline phosphatase and leucine aminopeptidase of isolated IEC are localized to the brush borders of IEC in tissue and in most newly isolated IEC. With time, both enzymes are found distributed over the entire cell surface. Redistribution appears to occur by diffusion in the plane of the membrane. It is slowed, but not blocked, if cells are maintained at 0° C instead of at 37° C, and it is not blocked by fixation in 0.5-3% paraformaldehyde. Drugs that alter cell membrane potential or that affect cell levels of ATP enhance the rate of redistribution of the enzymes.
UR - http://www.scopus.com/inward/record.url?scp=0019133697&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019133697&partnerID=8YFLogxK
U2 - 10.1083/jcb.86.3.849
DO - 10.1083/jcb.86.3.849
M3 - Article
C2 - 7410482
AN - SCOPUS:0019133697
SN - 0021-9525
VL - 86
SP - 849
EP - 857
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
ER -