Redistribution of membrane proteins in isolated mouse intestinal epithelial cells

Carol Ann Ziomek, Seth Schulman, Michael Edidin

Research output: Contribution to journalArticlepeer-review

Abstract

Single mouse intestinal epithelial cells (IEC) may be isolated by the use of a combination of methods used for the isolation of IEC from other species. Isolated cells remain viable for several hours. The membrane integral enzymes alkaline phosphatase and leucine aminopeptidase of isolated IEC are localized to the brush borders of IEC in tissue and in most newly isolated IEC. With time, both enzymes are found distributed over the entire cell surface. Redistribution appears to occur by diffusion in the plane of the membrane. It is slowed, but not blocked, if cells are maintained at 0° C instead of at 37° C, and it is not blocked by fixation in 0.5-3% paraformaldehyde. Drugs that alter cell membrane potential or that affect cell levels of ATP enhance the rate of redistribution of the enzymes.

Original languageEnglish (US)
Pages (from-to)849-857
Number of pages9
JournalJournal of Cell Biology
Volume86
Issue number3
DOIs
StatePublished - Sep 1 1980

ASJC Scopus subject areas

  • Cell Biology

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