Abstract
Vascular amyloidosis in Alzheimer's disease (AD) results in the exposure of red blood cells to β-amyloid fibrils (Aβ). The potential in vivo ramifications of this exposure have been investigated by injecting Aβ1-40 alone or Aβ-bound mouse red blood cells into the circulation of C57BL/6 mice. Results indicate that when Aβ1-40 is injected alone, a transient uptake of the fibrils by red blood cells occurs in vivo. When Aβ-bound red blood cells were injected, β-amyloid is rapidly removed from these cells in vivo. Double-labeling experiments indicate that while some of the red blood cells bound to Aβ1-40 are removed from circulation, a major fraction of these cells remain in circulation even after Aβ is removed. Immunohistochemistry of murine tissue samples obtained after sacrificing the animals suggests that within 1 h after injection of Aβ1-40 or Aβ-bound red blood cells, Aβ is found in spleen phagocytes and liver Kupffer cells. Heme staining further indicates that the binding of Aβ1-40 to red blood cells enhances red cell phagocytosis by the spleen.
Original language | English (US) |
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Pages (from-to) | 28-37 |
Number of pages | 10 |
Journal | Neurobiology of Disease |
Volume | 19 |
Issue number | 1-2 |
DOIs | |
State | Published - 2005 |
Externally published | Yes |
Keywords
- Alzheimer's disease
- Erythrocytes
- Immunohistochemistry
- Liver
- Murine in vivo study
- Spleen
- β-Amyloid
ASJC Scopus subject areas
- Neurology