Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer

Scott A. Tomlins, Daniel R. Rhodes, Sven Perner, Saravana M. Dhanasekaran, Rohit Mehra, Xiao Wei Sun, Sooryanarayana Varambally, Xuhong Cao, Joelle Tchinda, Rainer Kuefer, Charles Lee, James E. Montie, Rajal B. Shah, Kenneth Pienta, Mark A. Rubin, Arul M. Chinnaiyan

Research output: Contribution to journalArticle

Abstract

Recurrent chromosomal rearrangements have not been well characterized in common carcinomas. We used a bioinformatics approach to discover candidate oncogenic chromosomal aberrations on the basis of outlier gene expression. Two ETS transcription factors, ERG and ETV1, were identified as outliers in prostate cancer. We identified recurrent gene fusions of the 5′ untranslated region of TMPRSS2 to ERG or ETV1 in prostate cancer tissues with outlier expression. By using fluorescence in situ hybridization, we demonstrated that 23 of 29 prostate cancer samples harbor rearrangements in ERG or ETV1. Cell line experiments suggest that the androgen-responsive promoter elements of TMPRSS2 mediate the overexpression of ETS family members in prostate cancer. These results have implications in the development of carcinomas and the molecular diagnosis and treatment of prostate cancer.

Original languageEnglish (US)
Pages (from-to)644-648
Number of pages5
JournalScience
Volume310
Issue number5748
DOIs
StatePublished - Oct 28 2005
Externally publishedYes

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Prostatic Neoplasms
Transcription Factors
Genes
Carcinoma
5' Untranslated Regions
Gene Fusion
Computational Biology
Fluorescence In Situ Hybridization
Chromosome Aberrations
Androgens
Gene Expression
Cell Line

ASJC Scopus subject areas

  • General

Cite this

Tomlins, S. A., Rhodes, D. R., Perner, S., Dhanasekaran, S. M., Mehra, R., Sun, X. W., ... Chinnaiyan, A. M. (2005). Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science, 310(5748), 644-648. https://doi.org/10.1126/science.1117679

Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. / Tomlins, Scott A.; Rhodes, Daniel R.; Perner, Sven; Dhanasekaran, Saravana M.; Mehra, Rohit; Sun, Xiao Wei; Varambally, Sooryanarayana; Cao, Xuhong; Tchinda, Joelle; Kuefer, Rainer; Lee, Charles; Montie, James E.; Shah, Rajal B.; Pienta, Kenneth; Rubin, Mark A.; Chinnaiyan, Arul M.

In: Science, Vol. 310, No. 5748, 28.10.2005, p. 644-648.

Research output: Contribution to journalArticle

Tomlins, SA, Rhodes, DR, Perner, S, Dhanasekaran, SM, Mehra, R, Sun, XW, Varambally, S, Cao, X, Tchinda, J, Kuefer, R, Lee, C, Montie, JE, Shah, RB, Pienta, K, Rubin, MA & Chinnaiyan, AM 2005, 'Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer', Science, vol. 310, no. 5748, pp. 644-648. https://doi.org/10.1126/science.1117679
Tomlins SA, Rhodes DR, Perner S, Dhanasekaran SM, Mehra R, Sun XW et al. Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science. 2005 Oct 28;310(5748):644-648. https://doi.org/10.1126/science.1117679
Tomlins, Scott A. ; Rhodes, Daniel R. ; Perner, Sven ; Dhanasekaran, Saravana M. ; Mehra, Rohit ; Sun, Xiao Wei ; Varambally, Sooryanarayana ; Cao, Xuhong ; Tchinda, Joelle ; Kuefer, Rainer ; Lee, Charles ; Montie, James E. ; Shah, Rajal B. ; Pienta, Kenneth ; Rubin, Mark A. ; Chinnaiyan, Arul M. / Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. In: Science. 2005 ; Vol. 310, No. 5748. pp. 644-648.
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