Recruitment of renin gene-expressing cells in adult rat kidneys

R. Ariel Gomez, Robert L. Chevalier, Allen D Everett, John P. Elwood, Michael J. Peach, Kevin R. Lynch, Robert M. Carey

Research output: Contribution to journalArticle

Abstract

To define whether angiotensin I-converting enzyme (ACE) inhibition affects the distribution of renin gene-expressing cells within the kidney, a control group of adult male Wistar-Kyoto rats (C, n = 7) was compared with a group of rats treated with enalapril (E, n = 6) for 5 days. Renin mRNA distribution was assessed using in situ hybridization to a 35S-labeled 28 mer oligonucleotide complementary to rat renin mRNA. Whereas in control rats renin mRNA was confined to a juxtaglomerular location, in enalapril-treated rats, renin mRNA extended proximally along the length of the afferent arteriole. The percent of visible afferent arteriolar length containing renin mRNA was higher in enalapril-treated (71.7 ± 2.8%) than in control (49.6 ± 2.1%) rats (P <0.0001). These findings were accompanied by an increase in the percent of juxtaglomerular apparatuses (JGAs) containing renin mRNA (71 ± 2.2 vs. 49 ± 2.9%; E vs. C, P <0.0001). Also, the intensity of the JGA hybridization signals was higher in enalapril-treated (757 ± 59 grains/JGA) than in control (167 ± 11 grains/JGA) rats (P <0.00001). We conclude that the increased kidney renin gene expression elicited by ACE inhibition is the result of an increase in renin mRNA content per JGA, an increase in the number of JGAs expressing the renin gene, and a recruitment of renin gene-expressing cells along the afferent arteriole.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume259
Issue number4 28-4
StatePublished - 1990
Externally publishedYes

Fingerprint

Renin
Juxtaglomerular Apparatus
Kidney
Genes
Enalapril
Messenger RNA
Arterioles
Peptidyl-Dipeptidase A
Inbred WKY Rats
Oligonucleotides
In Situ Hybridization
Gene Expression
Control Groups

Keywords

  • Angiotensin-converting enzyme
  • Enalapril
  • In situ hybridization
  • Intrarenal renin-angiotensin system
  • Messenger ribonucleic acid
  • Wistar-Kyoto rat

ASJC Scopus subject areas

  • Physiology

Cite this

Gomez, R. A., Chevalier, R. L., Everett, A. D., Elwood, J. P., Peach, M. J., Lynch, K. R., & Carey, R. M. (1990). Recruitment of renin gene-expressing cells in adult rat kidneys. American Journal of Physiology - Renal Fluid and Electrolyte Physiology, 259(4 28-4).

Recruitment of renin gene-expressing cells in adult rat kidneys. / Gomez, R. Ariel; Chevalier, Robert L.; Everett, Allen D; Elwood, John P.; Peach, Michael J.; Lynch, Kevin R.; Carey, Robert M.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 259, No. 4 28-4, 1990.

Research output: Contribution to journalArticle

Gomez, RA, Chevalier, RL, Everett, AD, Elwood, JP, Peach, MJ, Lynch, KR & Carey, RM 1990, 'Recruitment of renin gene-expressing cells in adult rat kidneys', American Journal of Physiology - Renal Fluid and Electrolyte Physiology, vol. 259, no. 4 28-4.
Gomez, R. Ariel ; Chevalier, Robert L. ; Everett, Allen D ; Elwood, John P. ; Peach, Michael J. ; Lynch, Kevin R. ; Carey, Robert M. / Recruitment of renin gene-expressing cells in adult rat kidneys. In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 1990 ; Vol. 259, No. 4 28-4.
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