Recruitment of CXCR3+ and CCR5+ T cells and production of interferon-γ-inducible chemokines in rejecting human arteries

William Burns, Yinong Wang, Paul C.Y. Tang, Hooman Ranjbaran, Alexander Iakimov, Jinah Kim, Madison Cuffy, Yalai Bai, Jordan S. Pober, George Tellides

Research output: Contribution to journalArticle

Abstract

Chemokine receptors preferentially expressed by Th1 cells and their IFN-γ-inducible ligands predominate in experimental and clinical allograft rejection. Previous chemokine-related transplantation studies have focused on parenchymal and microvascular inflammation which are of importance in acute rejection, but are not necessarily relevant in immune-mediated injury of conduit arteries. We have recently described a model of progressive human T cell-mediated infiltration and injury of allogeneic coronary artery segments using immunodeficient mouse hosts. In the present study, we investigated if recruitment of allogeneic T cells to different vascular compartments correlated with the expression of chemokines and their receptors. Transcripts were quantified by laser capture microdissection/real-time RT-PCR and their distribution was correlated to the corresponding protein expression detected by immunohistochemistry. Infiltrating T cells, confined to the adventitia and intima, expressed CXCR3 and CCR5, but were not recruited into the media despite production by vascular smooth muscle cells of IP-10, Mig, I-TAC, RANTES and MIP-1β. Chemokine mRNA was detected primarily in vascular cells, although chemokine protein largely localized to infiltrating leukocytes which uniquely expressed their cognate receptors. These data explain the recruitment of IFN-γ-secreting T cells to the vessel wall, and reinforce the suggestion that the arterial media may be a site of immunological privilege.

Original languageEnglish (US)
Pages (from-to)1226-1236
Number of pages11
JournalAmerican Journal of Transplantation
Volume5
Issue number6
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

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Chemokines
Interferons
Arteries
T-Lymphocytes
Chemokine Receptors
Blood Vessels
Laser Capture Microdissection
Tunica Media
Chemokine CCL5
Adventitia
Th1 Cells
Wounds and Injuries
Vascular Smooth Muscle
Smooth Muscle Myocytes
Allografts
Real-Time Polymerase Chain Reaction
Coronary Vessels
Proteins
Leukocytes
Transplantation

Keywords

  • Arteriosclerosis
  • Artery
  • Chemokines
  • T cells
  • Vascular remodeling

ASJC Scopus subject areas

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

Cite this

Recruitment of CXCR3+ and CCR5+ T cells and production of interferon-γ-inducible chemokines in rejecting human arteries. / Burns, William; Wang, Yinong; Tang, Paul C.Y.; Ranjbaran, Hooman; Iakimov, Alexander; Kim, Jinah; Cuffy, Madison; Bai, Yalai; Pober, Jordan S.; Tellides, George.

In: American Journal of Transplantation, Vol. 5, No. 6, 01.06.2005, p. 1226-1236.

Research output: Contribution to journalArticle

Burns, William ; Wang, Yinong ; Tang, Paul C.Y. ; Ranjbaran, Hooman ; Iakimov, Alexander ; Kim, Jinah ; Cuffy, Madison ; Bai, Yalai ; Pober, Jordan S. ; Tellides, George. / Recruitment of CXCR3+ and CCR5+ T cells and production of interferon-γ-inducible chemokines in rejecting human arteries. In: American Journal of Transplantation. 2005 ; Vol. 5, No. 6. pp. 1226-1236.
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