Recovery of waterborne Cryptosporidium parvum oocysts by freshwater benthic clams (Corbicula fluminea)

Thaddeus K. Graczyk, Ronald Fayer, Michael R. Cranfield, David Bruce Conn

Research output: Contribution to journalArticle

Abstract

Asian freshwater clams, Corbicula fluminea, exposed for 24 h to 38 liters of water contaminated with infectious Cryptosporidium parvum oocysts (1.00 x 106 oocysts/liter, approximately 1.9 x 105 oocysts/clam) were examined (hemolymph, gills, gastrointestinal [GI] tract, and feces) on days 1, 2, 3, 7, and 14 postexposure (PE). No oocysts were detected in the water 24 h after the contamination event. The percentage of oocyst-containing clams varied from 20 to 100%, depending on the type of tissue examined and the technique used-acid-fast stain (AFS) or immunofluorescent antibody (IFA). The oocysts were found in clam tissues and feces on days 1 through 14 PE; the oocysts extracted from the tissues on day 7 PE were infectious for neonatal BALB/c mice. Overall, the highest number of positive samples was obtained when gills and GI tracts were processed with IFA (prevalence, 97.5%). A comparison of the relative oocyst numbers indicated that overall, 58.3% of the oocysts were found in clam tissues and 41.7% were found in feces when IFA was used; when AFS was used, the values were 51.9 and 48.1%, respectively. Clam-released oocysts were always surrounded by feces; no free oocysts or oocysts disassociated from fecal matter were observed. The results indicate that these benthic freshwater clams are capable of recovery and sedimentation of waterborne C. parvum oocysts. To optimize the detection of C. parvum oocysts in C. fluminea tissue, it is recommended that gill and GI tract samples be screened with IFA (such as that in the commercially available MERIFLUOR test kit).

Original languageEnglish (US)
Pages (from-to)427-430
Number of pages4
JournalApplied and Environmental Microbiology
Volume64
Issue number2
StatePublished - Feb 1998

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Corbicula
Corbicula fluminea
Cryptosporidium parvum
Oocysts
Fresh Water
oocysts
feces
antibody
Feces
acid
gastrointestinal system
Gastrointestinal Tract
gills
Antibodies
tissue
sedimentation
antibodies
water
Coloring Agents
Acids

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Recovery of waterborne Cryptosporidium parvum oocysts by freshwater benthic clams (Corbicula fluminea). / Graczyk, Thaddeus K.; Fayer, Ronald; Cranfield, Michael R.; Conn, David Bruce.

In: Applied and Environmental Microbiology, Vol. 64, No. 2, 02.1998, p. 427-430.

Research output: Contribution to journalArticle

Graczyk, Thaddeus K. ; Fayer, Ronald ; Cranfield, Michael R. ; Conn, David Bruce. / Recovery of waterborne Cryptosporidium parvum oocysts by freshwater benthic clams (Corbicula fluminea). In: Applied and Environmental Microbiology. 1998 ; Vol. 64, No. 2. pp. 427-430.
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abstract = "Asian freshwater clams, Corbicula fluminea, exposed for 24 h to 38 liters of water contaminated with infectious Cryptosporidium parvum oocysts (1.00 x 106 oocysts/liter, approximately 1.9 x 105 oocysts/clam) were examined (hemolymph, gills, gastrointestinal [GI] tract, and feces) on days 1, 2, 3, 7, and 14 postexposure (PE). No oocysts were detected in the water 24 h after the contamination event. The percentage of oocyst-containing clams varied from 20 to 100{\%}, depending on the type of tissue examined and the technique used-acid-fast stain (AFS) or immunofluorescent antibody (IFA). The oocysts were found in clam tissues and feces on days 1 through 14 PE; the oocysts extracted from the tissues on day 7 PE were infectious for neonatal BALB/c mice. Overall, the highest number of positive samples was obtained when gills and GI tracts were processed with IFA (prevalence, 97.5{\%}). A comparison of the relative oocyst numbers indicated that overall, 58.3{\%} of the oocysts were found in clam tissues and 41.7{\%} were found in feces when IFA was used; when AFS was used, the values were 51.9 and 48.1{\%}, respectively. Clam-released oocysts were always surrounded by feces; no free oocysts or oocysts disassociated from fecal matter were observed. The results indicate that these benthic freshwater clams are capable of recovery and sedimentation of waterborne C. parvum oocysts. To optimize the detection of C. parvum oocysts in C. fluminea tissue, it is recommended that gill and GI tract samples be screened with IFA (such as that in the commercially available MERIFLUOR test kit).",
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