Recovery of the simian immunodeficiency virus (SIV) and depression of colony formation in in vitro infected progenitor cell-enriched rhesus bone marrow (BM).

L. Beltz, O. Narayan, Robert John Adams, S. J. Noga, A. D. Donnenberg

Research output: Contribution to journalArticle

Abstract

Rhesus progenitor-enriched BM was exposed overnight to SIV and cultured in a limiting dilution assay where the potential for progenitor interaction with lymphocytes or macrophages was low. Virus was consistently isolated late in culture, detection being aided by coculture with CEM174 lymphoblasts. Although infected cells had reduced clonogenic activity, colonies were indistinguishable from those derived from uninfected BM with respect to proliferative potential, morphology, and longevity in culture. Primate immunodeficiency viruses, therefore, may infect immature BM populations, directly affecting hematopoietic activity.

Original languageEnglish (US)
Pages (from-to)144-151
Number of pages8
JournalJournal of Medical Primatology
Volume20
Issue number4
StatePublished - Jun 1991

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
bone marrow
stem cells
Stem Cells
Bone Marrow
Primate Lentiviruses
viruses
immunosuppression
coculture
Coculture Techniques
Primates
macrophages
lymphocytes
immatures
Macrophages
Lymphocytes
Viruses
assays
Population

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Recovery of the simian immunodeficiency virus (SIV) and depression of colony formation in in vitro infected progenitor cell-enriched rhesus bone marrow (BM). / Beltz, L.; Narayan, O.; Adams, Robert John; Noga, S. J.; Donnenberg, A. D.

In: Journal of Medical Primatology, Vol. 20, No. 4, 06.1991, p. 144-151.

Research output: Contribution to journalArticle

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