Recovery of protein from tear fluid stored in cellulose sponges

Eric J. van Agtmaal, Nicolaas J. van Haeringen, Martin W. Bloem, Wil H.P. Schreurs, Sastri Saowakontha

Research output: Contribution to journalArticlepeer-review

Abstract

Human tear fluid collected in glass capillaries was absorbed in cellulose sponges. After various time intervals the recovery was measured for total protein, amylase (AMY), lysozyme (LZM), immunoglobulin A (IgA) and lactoferrin (LF). After one day storage at 4°C of moistened sponges of at least 92% recovery was observed. Storage for longer periods at 4°C up to seven days showed a decreasing recovery for total protein, AMY and IgA. Tear collection with sponges and storage of wet sponges for maximally one day at 4°C followed by centrifugation or storage of the fluid at-20°C are reliable ways of sample treatment.

Original languageEnglish (US)
Pages (from-to)585-588
Number of pages4
JournalCurrent Eye Research
Volume6
Issue number4
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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