Recovery of protein from tear fluid stored in cellulose sponges

Eric J. van Agtmaal, Nicolaas J. van Haeringen, Martin Bloem, Wil H P Schreurs, Sastri Saowakontha

Research output: Contribution to journalArticle

Abstract

Human tear fluid collected in glass capillaries was absorbed in cellulose sponges. After various time intervals the recovery was measured for total protein, amylase (AMY), lysozyme (LZM), immunoglobulin A (IgA) and lactoferrin (LF). After one day storage at 4°C of moistened sponges of at least 92% recovery was observed. Storage for longer periods at 4°C up to seven days showed a decreasing recovery for total protein, AMY and IgA. Tear collection with sponges and storage of wet sponges for maximally one day at 4°C followed by centrifugation or storage of the fluid at-20°C are reliable ways of sample treatment.

Original languageEnglish (US)
Pages (from-to)585-588
Number of pages4
JournalCurrent Eye Research
Volume6
Issue number4
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Porifera
Cellulose
Amylases
Tears
Immunoglobulin A
Lactoferrin
Staphylococcal Protein A
Muramidase
Centrifugation
Glass
tear proteins
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

van Agtmaal, E. J., van Haeringen, N. J., Bloem, M., Schreurs, W. H. P., & Saowakontha, S. (1987). Recovery of protein from tear fluid stored in cellulose sponges. Current Eye Research, 6(4), 585-588. https://doi.org/10.3109/02713688709025217

Recovery of protein from tear fluid stored in cellulose sponges. / van Agtmaal, Eric J.; van Haeringen, Nicolaas J.; Bloem, Martin; Schreurs, Wil H P; Saowakontha, Sastri.

In: Current Eye Research, Vol. 6, No. 4, 1987, p. 585-588.

Research output: Contribution to journalArticle

van Agtmaal, EJ, van Haeringen, NJ, Bloem, M, Schreurs, WHP & Saowakontha, S 1987, 'Recovery of protein from tear fluid stored in cellulose sponges', Current Eye Research, vol. 6, no. 4, pp. 585-588. https://doi.org/10.3109/02713688709025217
van Agtmaal, Eric J. ; van Haeringen, Nicolaas J. ; Bloem, Martin ; Schreurs, Wil H P ; Saowakontha, Sastri. / Recovery of protein from tear fluid stored in cellulose sponges. In: Current Eye Research. 1987 ; Vol. 6, No. 4. pp. 585-588.
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