Recovery of Histoplasma capsulatum from blood in a commercial radiometric mycobacterium medium

W. G. Merz, S. Kodsy, C. S. Merz

Research output: Contribution to journalArticle

Abstract

We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabolic signals occurred at a mean of 11 days, but no organisms were detected with acid-fast stains. The bottles remained positive, and after an additional incubation (mean, 8 days), yeast cells morphologically compatible with H. capsulatum were detected when aliquots were stained with acridine orange. Therefore, when radiometric mycobacterial blood cultures with persistent positive metabolic signals and negative acid-fast stains are encountered, acridine orange staining and subculturing for a variety of microorganisms, including fungi, e.g., H. capsulatum, should be considered.

Original languageEnglish (US)
Pages (from-to)237-239
Number of pages3
JournalJournal of Clinical Microbiology
Volume30
Issue number1
StatePublished - 1992

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Histoplasma
Mycobacterium
Acridine Orange
Coloring Agents
Acids
Fungi
Yeasts
HIV
Staining and Labeling

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Recovery of Histoplasma capsulatum from blood in a commercial radiometric mycobacterium medium. / Merz, W. G.; Kodsy, S.; Merz, C. S.

In: Journal of Clinical Microbiology, Vol. 30, No. 1, 1992, p. 237-239.

Research output: Contribution to journalArticle

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