Recording membrane potential changes through photoacoustic voltage sensitive dye

Haichong K. Zhang, Jeeun Kang, Ping Yan, Diane S. Abou, Hanh N.D. Le, Daniel L.J. Thorek, Jin U. Kang, Albert Gjedde, Arman Rahmim, Dean F. Wong, Leslie M. Loew, Emad M. Boctor

Research output: ResearchConference contribution

Abstract

Monitoring of the membrane potential is possible using voltage sensitive dyes (VSD), where fluorescence intensity changes in response to neuronal electrical activity. However, fluorescence imaging is limited by depth of penetration and high scattering losses, which leads to low sensitivity in vivo systems for external detection. In contrast, photoacoustic (PA) imaging, an emerging modality, is capable of deep tissue, noninvasive imaging by combining near infrared light excitation and ultrasound detection. In this work, we develop the theoretical concept whereby the voltage-dependent quenching of dye fluorescence leads to a reciprocal enhancement of PA intensity. Based on this concept, we synthesized a novel near infrared photoacoustic VSD (PA-VSD) whose PA intensity change is sensitive to membrane potential. In the polarized state, this cyanine-based probe enhances PA intensity while decreasing fluorescence output in a lipid vesicle membrane model. With a 3-9 μM VSD concentration, we measured a PA signal increase in the range of 5.3 % to 18.1 %, and observed a corresponding signal reduction in fluorescence emission of 30.0 % to 48.7 %. A theoretical model successfully accounts for how the experimental PA intensity change depends on fluorescence and absorbance properties of the dye. These results not only demonstrate the voltage sensing capability of the dye, but also indicate the necessity of considering both fluorescence and absorbance spectral sensitivities in order to optimize the characteristics of improved photoacoustic probes. Together, our results demonstrate photoacoustic sensing as a potential new modality for sub-second recording and external imaging of electrophysiological and neurochemical events in the brain.

LanguageEnglish (US)
Title of host publicationPhotons Plus Ultrasound: Imaging and Sensing 2017
PublisherSPIE
Volume10064
ISBN (Electronic)9781510605695
DOIs
StatePublished - 2017
EventPhotons Plus Ultrasound: Imaging and Sensing 2017 - San Francisco, United States
Duration: Jan 29 2017Feb 1 2017

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2017
CountryUnited States
CitySan Francisco
Period1/29/172/1/17

Fingerprint

dyes
recording
membranes
fluorescence
electric potential
Photoacoustic effect
Coloring Agents
Membranes
Electric potential
Dyes
Membrane Potentials
Fluorescence
Imaging techniques
probes
Infrared radiation
Optical Imaging
Membrane Lipids
Theoretical Models
Light
Brain

Keywords

  • Fluorescence
  • Membrane potential
  • Photoacoustics
  • Voltage sensitive dye

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Zhang, H. K., Kang, J., Yan, P., Abou, D. S., Le, H. N. D., Thorek, D. L. J., ... Boctor, E. M. (2017). Recording membrane potential changes through photoacoustic voltage sensitive dye. In Photons Plus Ultrasound: Imaging and Sensing 2017 (Vol. 10064). [1006407] SPIE. DOI: 10.1117/12.2252442

Recording membrane potential changes through photoacoustic voltage sensitive dye. / Zhang, Haichong K.; Kang, Jeeun; Yan, Ping; Abou, Diane S.; Le, Hanh N.D.; Thorek, Daniel L.J.; Kang, Jin U.; Gjedde, Albert; Rahmim, Arman; Wong, Dean F.; Loew, Leslie M.; Boctor, Emad M.

Photons Plus Ultrasound: Imaging and Sensing 2017. Vol. 10064 SPIE, 2017. 1006407.

Research output: ResearchConference contribution

Zhang, HK, Kang, J, Yan, P, Abou, DS, Le, HND, Thorek, DLJ, Kang, JU, Gjedde, A, Rahmim, A, Wong, DF, Loew, LM & Boctor, EM 2017, Recording membrane potential changes through photoacoustic voltage sensitive dye. in Photons Plus Ultrasound: Imaging and Sensing 2017. vol. 10064, 1006407, SPIE, Photons Plus Ultrasound: Imaging and Sensing 2017, San Francisco, United States, 1/29/17. DOI: 10.1117/12.2252442
Zhang HK, Kang J, Yan P, Abou DS, Le HND, Thorek DLJ et al. Recording membrane potential changes through photoacoustic voltage sensitive dye. In Photons Plus Ultrasound: Imaging and Sensing 2017. Vol. 10064. SPIE. 2017. 1006407. Available from, DOI: 10.1117/12.2252442
Zhang, Haichong K. ; Kang, Jeeun ; Yan, Ping ; Abou, Diane S. ; Le, Hanh N.D. ; Thorek, Daniel L.J. ; Kang, Jin U. ; Gjedde, Albert ; Rahmim, Arman ; Wong, Dean F. ; Loew, Leslie M. ; Boctor, Emad M./ Recording membrane potential changes through photoacoustic voltage sensitive dye. Photons Plus Ultrasound: Imaging and Sensing 2017. Vol. 10064 SPIE, 2017.
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