Reconstruction of functionally normal and malignant human breast tissues in mice

Charlotte Kuperwasser, Tony Chavarria, Min Wu, Greg Magrane, Joe W. Gray, Loucinda Carey, Andrea Richardson, Robert A. Weinberg

Research output: Contribution to journalArticle

Abstract

The study of normal breast epithelial morphogenesis and carcinogenesis in vivo has largely used rodent models. Efforts at studying mammary morphogenesis and cancer with xenotransplanted human epithelial cells have failed to recapitulate the full extent of development seen in the human breast. We have developed an orthotopic xenograft model in which both the stromal and epithelial components of the reconstructed mammary gland are of human origin. Genetic modification of human stromal cells before the implantation of ostensibly normal human mammary epithelial cells resulted in the outgrowth of benign and malignant lesions. This experimental model allows for studies of human epithelial morphogenesis and differentiation in vivo and underscores the critical role of heterotypic interactions in human breast development and carcinogenesis.

Original languageEnglish (US)
Pages (from-to)4966-4971
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number14
DOIs
StatePublished - Apr 6 2004
Externally publishedYes

Fingerprint

Breast
Morphogenesis
Carcinogenesis
Epithelial Cells
Medical Genetics
Human Development
Human Mammary Glands
Stromal Cells
Heterografts
Rodentia
Theoretical Models
Breast Neoplasms

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Reconstruction of functionally normal and malignant human breast tissues in mice. / Kuperwasser, Charlotte; Chavarria, Tony; Wu, Min; Magrane, Greg; Gray, Joe W.; Carey, Loucinda; Richardson, Andrea; Weinberg, Robert A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 14, 06.04.2004, p. 4966-4971.

Research output: Contribution to journalArticle

Kuperwasser, Charlotte ; Chavarria, Tony ; Wu, Min ; Magrane, Greg ; Gray, Joe W. ; Carey, Loucinda ; Richardson, Andrea ; Weinberg, Robert A. / Reconstruction of functionally normal and malignant human breast tissues in mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 14. pp. 4966-4971.
@article{efac641e2c994b4fbf1eb711691fb427,
title = "Reconstruction of functionally normal and malignant human breast tissues in mice",
abstract = "The study of normal breast epithelial morphogenesis and carcinogenesis in vivo has largely used rodent models. Efforts at studying mammary morphogenesis and cancer with xenotransplanted human epithelial cells have failed to recapitulate the full extent of development seen in the human breast. We have developed an orthotopic xenograft model in which both the stromal and epithelial components of the reconstructed mammary gland are of human origin. Genetic modification of human stromal cells before the implantation of ostensibly normal human mammary epithelial cells resulted in the outgrowth of benign and malignant lesions. This experimental model allows for studies of human epithelial morphogenesis and differentiation in vivo and underscores the critical role of heterotypic interactions in human breast development and carcinogenesis.",
author = "Charlotte Kuperwasser and Tony Chavarria and Min Wu and Greg Magrane and Gray, {Joe W.} and Loucinda Carey and Andrea Richardson and Weinberg, {Robert A.}",
year = "2004",
month = "4",
day = "6",
doi = "10.1073/pnas.0401064101",
language = "English (US)",
volume = "101",
pages = "4966--4971",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "14",

}

TY - JOUR

T1 - Reconstruction of functionally normal and malignant human breast tissues in mice

AU - Kuperwasser, Charlotte

AU - Chavarria, Tony

AU - Wu, Min

AU - Magrane, Greg

AU - Gray, Joe W.

AU - Carey, Loucinda

AU - Richardson, Andrea

AU - Weinberg, Robert A.

PY - 2004/4/6

Y1 - 2004/4/6

N2 - The study of normal breast epithelial morphogenesis and carcinogenesis in vivo has largely used rodent models. Efforts at studying mammary morphogenesis and cancer with xenotransplanted human epithelial cells have failed to recapitulate the full extent of development seen in the human breast. We have developed an orthotopic xenograft model in which both the stromal and epithelial components of the reconstructed mammary gland are of human origin. Genetic modification of human stromal cells before the implantation of ostensibly normal human mammary epithelial cells resulted in the outgrowth of benign and malignant lesions. This experimental model allows for studies of human epithelial morphogenesis and differentiation in vivo and underscores the critical role of heterotypic interactions in human breast development and carcinogenesis.

AB - The study of normal breast epithelial morphogenesis and carcinogenesis in vivo has largely used rodent models. Efforts at studying mammary morphogenesis and cancer with xenotransplanted human epithelial cells have failed to recapitulate the full extent of development seen in the human breast. We have developed an orthotopic xenograft model in which both the stromal and epithelial components of the reconstructed mammary gland are of human origin. Genetic modification of human stromal cells before the implantation of ostensibly normal human mammary epithelial cells resulted in the outgrowth of benign and malignant lesions. This experimental model allows for studies of human epithelial morphogenesis and differentiation in vivo and underscores the critical role of heterotypic interactions in human breast development and carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=1842687362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842687362&partnerID=8YFLogxK

U2 - 10.1073/pnas.0401064101

DO - 10.1073/pnas.0401064101

M3 - Article

C2 - 15051869

AN - SCOPUS:1842687362

VL - 101

SP - 4966

EP - 4971

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 14

ER -