Reconstitution of the Kaposi's sarcoma-associated herpesvirus nuclear egress complex and formation of nuclear membrane vesicles by coexpression of ORF67 and ORF69 gene products

Prashant J. Desai; Erin N. Pryce; Brandon W. Henson; Eric M. Luitweiler; Jonathan Cothran

doi:10.1128/JVI.05988-11

Reconstitution of the Kaposi's sarcoma-associated herpesvirus nuclear egress complex and formation of nuclear membrane vesicles by coexpression of ORF67 and ORF69 gene products

Prashant J. Desai, Erin N. Pryce, Brandon W. Henson, Eric M. Luitweiler, Jonathan Cothran

School of Medicine

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48 Scopus citations

Abstract

The Kaposi's sarcoma-associated herpesvirus nuclear egress complex is composed of two proteins, ORF67 and ORF69. In this study, we have recapitulated the KSHV complex by coexpression of these two proteins in insect cells using expression from recombinant baculoviruses. The proteins form a complex at the nuclear membrane as judged by live-cell analysis of protein fusions tagged with green uorescent protein (GFP) and mCherry. Ultrastructural analysis of infected cells showed that ORF67 expression results in reduplication of the nuclear membrane.When the two proteins are expressed together, numerous virion-size nuclear membrane-derived vesicles were evident at the nuclear margins.

Original language	English (US)
Pages (from-to)	594-598
Number of pages	5
Journal	Journal of virology
Volume	86
Issue number	1
DOIs	https://doi.org/10.1128/JVI.05988-11
State	Published - Jan 2012

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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Reconstitution of the Kaposi's sarcoma-associated herpesvirus nuclear egress complex and formation of nuclear membrane vesicles by coexpression of ORF67 and ORF69 gene products. / Desai, Prashant J.; Pryce, Erin N.; Henson, Brandon W. et al.

In: Journal of virology, Vol. 86, No. 1, 01.2012, p. 594-598.

Research output: Contribution to journal › Article › peer-review

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Reconstitution of the Kaposi's sarcoma-associated herpesvirus nuclear egress complex and formation of nuclear membrane vesicles by coexpression of ORF67 and ORF69 gene products

Abstract

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