Reconstitution of phosphate-linked antiport from Streptococcus,lactis

Suresh V. Ambudkar; Peter C. Maloney

doi:10.1016/0006-291X(85)90189-5

Reconstitution of phosphate-linked antiport from Streptococcus,lactis

Suresh V. Ambudkar, Peter C. Maloney

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Membrane protein solubilized by octyl-β-D-glucopyranoside in the presence of dispersed phospholipid was incubated with bath-sonicated liposomes and additional detergent. The proteoliposomes formed on dilution showed transport and exchange properties consistent with a reconstitution of phosphate: sugar 6-phosphate antiport. Thus, phosphate self-exchange was found only when protein from induced cells was used; this exchange was blocked by a sugar 6-phosphate, not by a sugar 1-phosphate; and proteoliposomes supported an accumulation of 2-deoxyglucose 6-phosphate with no added source of energy. Solubilization and reconstitution of protein was most effective when performed in the presence of gram-positive phospholipids.

Original language	English (US)
Pages (from-to)	568-575
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	129
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(85)90189-5
State	Published - Jun 14 1985
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Reconstitution of phosphate-linked antiport from Streptococcus,lactis",

abstract = "Membrane protein solubilized by octyl-β-D-glucopyranoside in the presence of dispersed phospholipid was incubated with bath-sonicated liposomes and additional detergent. The proteoliposomes formed on dilution showed transport and exchange properties consistent with a reconstitution of phosphate: sugar 6-phosphate antiport. Thus, phosphate self-exchange was found only when protein from induced cells was used; this exchange was blocked by a sugar 6-phosphate, not by a sugar 1-phosphate; and proteoliposomes supported an accumulation of 2-deoxyglucose 6-phosphate with no added source of energy. Solubilization and reconstitution of protein was most effective when performed in the presence of gram-positive phospholipids.",

author = "Ambudkar, {Suresh V.} and Maloney, {Peter C.}",

note = "Funding Information: to thank Drs. C.-C. Chen and T.H. Wilson for the generous gift washed asolectin, and for advice concerning reconstitution. to thank Dr. S. Krag and Mr. J.B. Fanning, Jr. for their help in phospholipids from S. lactis. This work was supported by grant the United States PLblic Health Service. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1985",

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doi = "10.1016/0006-291X(85)90189-5",

language = "English (US)",

volume = "129",

pages = "568--575",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Reconstitution of phosphate-linked antiport from Streptococcus,lactis

AU - Ambudkar, Suresh V.

AU - Maloney, Peter C.

N1 - Funding Information: to thank Drs. C.-C. Chen and T.H. Wilson for the generous gift washed asolectin, and for advice concerning reconstitution. to thank Dr. S. Krag and Mr. J.B. Fanning, Jr. for their help in phospholipids from S. lactis. This work was supported by grant the United States PLblic Health Service. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1985/6/14

Y1 - 1985/6/14

N2 - Membrane protein solubilized by octyl-β-D-glucopyranoside in the presence of dispersed phospholipid was incubated with bath-sonicated liposomes and additional detergent. The proteoliposomes formed on dilution showed transport and exchange properties consistent with a reconstitution of phosphate: sugar 6-phosphate antiport. Thus, phosphate self-exchange was found only when protein from induced cells was used; this exchange was blocked by a sugar 6-phosphate, not by a sugar 1-phosphate; and proteoliposomes supported an accumulation of 2-deoxyglucose 6-phosphate with no added source of energy. Solubilization and reconstitution of protein was most effective when performed in the presence of gram-positive phospholipids.

AB - Membrane protein solubilized by octyl-β-D-glucopyranoside in the presence of dispersed phospholipid was incubated with bath-sonicated liposomes and additional detergent. The proteoliposomes formed on dilution showed transport and exchange properties consistent with a reconstitution of phosphate: sugar 6-phosphate antiport. Thus, phosphate self-exchange was found only when protein from induced cells was used; this exchange was blocked by a sugar 6-phosphate, not by a sugar 1-phosphate; and proteoliposomes supported an accumulation of 2-deoxyglucose 6-phosphate with no added source of energy. Solubilization and reconstitution of protein was most effective when performed in the presence of gram-positive phospholipids.

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Reconstitution of phosphate-linked antiport from Streptococcus,lactis

Abstract

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