Reconstitution of phosphate-linked antiport from Streptococcus,lactis

Suresh V. Ambudkar, Peter C. Maloney

Research output: Contribution to journalArticlepeer-review

Abstract

Membrane protein solubilized by octyl-β-D-glucopyranoside in the presence of dispersed phospholipid was incubated with bath-sonicated liposomes and additional detergent. The proteoliposomes formed on dilution showed transport and exchange properties consistent with a reconstitution of phosphate: sugar 6-phosphate antiport. Thus, phosphate self-exchange was found only when protein from induced cells was used; this exchange was blocked by a sugar 6-phosphate, not by a sugar 1-phosphate; and proteoliposomes supported an accumulation of 2-deoxyglucose 6-phosphate with no added source of energy. Solubilization and reconstitution of protein was most effective when performed in the presence of gram-positive phospholipids.

Original languageEnglish (US)
Pages (from-to)568-575
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume129
Issue number2
DOIs
StatePublished - Jun 14 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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