Reconstituting the nuclear envelope and endoplasmic reticulum in vitro

Katherine L. Wilson, Christiane Wiese

Research output: Contribution to journalArticlepeer-review

Abstract

We describe how in-vitro reconstitution assays are being used to understand the assembly and structure of the nuclear envelope (NE) and endoplasmic reticulum (ER), focussing on membrane dynamics. Our review is partly historical, to honor the cell biologists who began reconstituting nuclear assembly in vitro about 14 years ago. These pioneering complex reconstitution experiments had an enormous impact on our ability to study both organelle assembly and cell cycle regulation. We will illustrate how cell-free exacts from a variety of organisms are now being used to define assembly and disassembly pathways, examine their cell cycle regulation, and test the roles of specific proteins in nuclear and ER formation.

Original languageEnglish (US)
Pages (from-to)487-496
Number of pages10
JournalSeminars in Cell and Developmental Biology
Volume7
Issue number4
DOIs
StatePublished - Aug 1996

Keywords

  • Assembly
  • Endoplasmic reticulum
  • Mitosis
  • Motors
  • Nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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