Reconstituted olfactory receptors in bilayer lipid membranes

Menekhem Zviman; H. Ti Tien

doi:10.1016/0302-4598(94)05016-N

Reconstituted olfactory receptors in bilayer lipid membranes

Menekhem Zviman, H. Ti Tien

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Bullfrog olfactory receptors were reconstituted in bilayer lipid membranes (BLMs). Three odorants were presented to the reconstituted system. The three structurally related odorants were diethylsulfide (DES), thiophene (THP) and diethanolsulfide (DOS). The ordorants were presented in pairs. DOS induced a response in the presence of either of the other two odorants. DES and THP did not induce a response in the presence of either of the other two odorants. These observations suggest that there are two substructures, one common to the three odorants and one that is unique to DOS. The results support the notion that olfactory receptors detect certain molecular segments of odorants.

Original language	English (US)
Pages (from-to)	127-135
Number of pages	9
Journal	Bioelectrochemistry and Bioenergetics
Volume	36
Issue number	2
DOIs	https://doi.org/10.1016/0302-4598(94)05016-N
State	Published - Mar 1995
Externally published	Yes

Keywords

Bilayer lipid membranes
Membrane receptor
Olfaction
Reconstitution

ASJC Scopus subject areas

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

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10.1016/0302-4598(94)05016-N

Cite this

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title = "Reconstituted olfactory receptors in bilayer lipid membranes",

abstract = "Bullfrog olfactory receptors were reconstituted in bilayer lipid membranes (BLMs). Three odorants were presented to the reconstituted system. The three structurally related odorants were diethylsulfide (DES), thiophene (THP) and diethanolsulfide (DOS). The ordorants were presented in pairs. DOS induced a response in the presence of either of the other two odorants. DES and THP did not induce a response in the presence of either of the other two odorants. These observations suggest that there are two substructures, one common to the three odorants and one that is unique to DOS. The results support the notion that olfactory receptors detect certain molecular segments of odorants.",

keywords = "Bilayer lipid membranes, Membrane receptor, Olfaction, Reconstitution",

author = "Menekhem Zviman and {Ti Tien}, H.",

note = "Funding Information: This work was supportedin part by NIH grant GM-147971a ndARO grantD AAL03-91-G-0062.",

year = "1995",

month = mar,

doi = "10.1016/0302-4598(94)05016-N",

language = "English (US)",

volume = "36",

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journal = "Bioelectrochemistry and Bioenergetics",

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T1 - Reconstituted olfactory receptors in bilayer lipid membranes

AU - Zviman, Menekhem

AU - Ti Tien, H.

N1 - Funding Information: This work was supportedin part by NIH grant GM-147971a ndARO grantD AAL03-91-G-0062.

PY - 1995/3

Y1 - 1995/3

N2 - Bullfrog olfactory receptors were reconstituted in bilayer lipid membranes (BLMs). Three odorants were presented to the reconstituted system. The three structurally related odorants were diethylsulfide (DES), thiophene (THP) and diethanolsulfide (DOS). The ordorants were presented in pairs. DOS induced a response in the presence of either of the other two odorants. DES and THP did not induce a response in the presence of either of the other two odorants. These observations suggest that there are two substructures, one common to the three odorants and one that is unique to DOS. The results support the notion that olfactory receptors detect certain molecular segments of odorants.

AB - Bullfrog olfactory receptors were reconstituted in bilayer lipid membranes (BLMs). Three odorants were presented to the reconstituted system. The three structurally related odorants were diethylsulfide (DES), thiophene (THP) and diethanolsulfide (DOS). The ordorants were presented in pairs. DOS induced a response in the presence of either of the other two odorants. DES and THP did not induce a response in the presence of either of the other two odorants. These observations suggest that there are two substructures, one common to the three odorants and one that is unique to DOS. The results support the notion that olfactory receptors detect certain molecular segments of odorants.

KW - Bilayer lipid membranes

KW - Membrane receptor

KW - Olfaction

KW - Reconstitution

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JO - Bioelectrochemistry and Bioenergetics

JF - Bioelectrochemistry and Bioenergetics

IS - 2

ER -

Reconstituted olfactory receptors in bilayer lipid membranes

Abstract

Keywords

ASJC Scopus subject areas

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