Recombinant virus vaccination against 'self' antigens using anchor- fixed immunogens

Kari R. Irvine; Maria R. Parkhurst; Eliza P. Shulman; Janis P. Tupesis; Mary Custer; Christopher E. Touloukian; Paul F. Robbins; Alicia Gómez Yafal; Patricia Greenhalgh; Roger P.M. Sutmuller; Rienk Offringa; Steven A. Rosenberg; Nicholas P. Restifo

Recombinant virus vaccination against 'self' antigens using anchor- fixed immunogens

Kari R. Irvine, Maria R. Parkhurst, Eliza P. Shulman, Janis P. Tupesis, Mary Custer, Christopher E. Touloukian, Paul F. Robbins, Alicia Gómez Yafal, Patricia Greenhalgh, Roger P.M. Sutmuller, Rienk Offringa, Steven A. Rosenberg, Nicholas P. Restifo

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

To study the induction of anti-'self' CD8⁺ T-cell reactivity against the tumor antigen gp100, we used a mouse transgenic for a chimeric HLAA*0201/H-2 K^b molecule (A2/K(b)). We immunized the mice with a recombinant vaccinia virus encoding a form of gp100 that had been modified at position 210 (from a threonine to a methionine) to increase epitope binding to the restricting class I molecule. Immunogens containing the 'anchor- fixed' modification elicited anti-self CD8⁺ T cells specific for the wild- type gp100_209-217 peptide pulsed onto target cells. More important, these cells specifically recognized the naturally presented epitope on the surface of an A2/K^b-expressing murine melanoma, B16. These data indicate that anchor-fixing epitopes could enhance the function of recombinant virus- based immunogens.

Original language	English (US)
Pages (from-to)	2536-2540
Number of pages	5
Journal	Cancer Research
Volume	59
Issue number	11
State	Published - Jun 1 1999
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Recombinant virus vaccination against 'self' antigens using anchor- fixed immunogens",

abstract = "To study the induction of anti-'self' CD8+ T-cell reactivity against the tumor antigen gp100, we used a mouse transgenic for a chimeric HLAA*0201/H-2 Kb molecule (A2/K(b)). We immunized the mice with a recombinant vaccinia virus encoding a form of gp100 that had been modified at position 210 (from a threonine to a methionine) to increase epitope binding to the restricting class I molecule. Immunogens containing the 'anchor- fixed' modification elicited anti-self CD8+ T cells specific for the wild- type gp100209-217 peptide pulsed onto target cells. More important, these cells specifically recognized the naturally presented epitope on the surface of an A2/Kb-expressing murine melanoma, B16. These data indicate that anchor-fixing epitopes could enhance the function of recombinant virus- based immunogens.",

author = "Irvine, {Kari R.} and Parkhurst, {Maria R.} and Shulman, {Eliza P.} and Tupesis, {Janis P.} and Mary Custer and Touloukian, {Christopher E.} and Robbins, {Paul F.} and {G{\'o}mez Yafal}, Alicia and Patricia Greenhalgh and Sutmuller, {Roger P.M.} and Rienk Offringa and Rosenberg, {Steven A.} and Restifo, {Nicholas P.}",

year = "1999",

month = jun,

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language = "English (US)",

volume = "59",

pages = "2536--2540",

journal = "Cancer Research",

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T1 - Recombinant virus vaccination against 'self' antigens using anchor- fixed immunogens

AU - Irvine, Kari R.

AU - Parkhurst, Maria R.

AU - Shulman, Eliza P.

AU - Tupesis, Janis P.

AU - Custer, Mary

AU - Touloukian, Christopher E.

AU - Robbins, Paul F.

AU - Gómez Yafal, Alicia

AU - Greenhalgh, Patricia

AU - Sutmuller, Roger P.M.

AU - Offringa, Rienk

AU - Rosenberg, Steven A.

AU - Restifo, Nicholas P.

PY - 1999/6/1

Y1 - 1999/6/1

N2 - To study the induction of anti-'self' CD8+ T-cell reactivity against the tumor antigen gp100, we used a mouse transgenic for a chimeric HLAA*0201/H-2 Kb molecule (A2/K(b)). We immunized the mice with a recombinant vaccinia virus encoding a form of gp100 that had been modified at position 210 (from a threonine to a methionine) to increase epitope binding to the restricting class I molecule. Immunogens containing the 'anchor- fixed' modification elicited anti-self CD8+ T cells specific for the wild- type gp100209-217 peptide pulsed onto target cells. More important, these cells specifically recognized the naturally presented epitope on the surface of an A2/Kb-expressing murine melanoma, B16. These data indicate that anchor-fixing epitopes could enhance the function of recombinant virus- based immunogens.

AB - To study the induction of anti-'self' CD8+ T-cell reactivity against the tumor antigen gp100, we used a mouse transgenic for a chimeric HLAA*0201/H-2 Kb molecule (A2/K(b)). We immunized the mice with a recombinant vaccinia virus encoding a form of gp100 that had been modified at position 210 (from a threonine to a methionine) to increase epitope binding to the restricting class I molecule. Immunogens containing the 'anchor- fixed' modification elicited anti-self CD8+ T cells specific for the wild- type gp100209-217 peptide pulsed onto target cells. More important, these cells specifically recognized the naturally presented epitope on the surface of an A2/Kb-expressing murine melanoma, B16. These data indicate that anchor-fixing epitopes could enhance the function of recombinant virus- based immunogens.

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JO - Cancer Research

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Recombinant virus vaccination against 'self' antigens using anchor- fixed immunogens

Abstract

ASJC Scopus subject areas

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