To study the induction of anti-'self' CD8+ T-cell reactivity against the tumor antigen gp100, we used a mouse transgenic for a chimeric HLAA*0201/H-2 Kb molecule (A2/K(b)). We immunized the mice with a recombinant vaccinia virus encoding a form of gp100 that had been modified at position 210 (from a threonine to a methionine) to increase epitope binding to the restricting class I molecule. Immunogens containing the 'anchor- fixed' modification elicited anti-self CD8+ T cells specific for the wild- type gp100209-217 peptide pulsed onto target cells. More important, these cells specifically recognized the naturally presented epitope on the surface of an A2/Kb-expressing murine melanoma, B16. These data indicate that anchor-fixing epitopes could enhance the function of recombinant virus- based immunogens.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Jun 1 1999|
ASJC Scopus subject areas
- Cancer Research