Recombinant interleukin 4 (rIL4) inhibits interleukin 2-induced activation of peripheral blood lymphocytes

X. Han, K. Itoh, Charles M. Balch, N. R. Pellis

Research output: Contribution to journalArticle

Abstract

Recombinant interleukin 4 (rIL4) inhibited the proliferation and induction of lymphokine activated killer (LAK) cells in human peripheral blood mononuclear cells (PBMC) cultured in recombinant interleukin 2 (rIL2). Other recombinant cytokines, such as rIFNγ, rTNFα, rIL1, and rIL3, did not inhibit LAK cell induction. rIL4-mediated suppression was dose dependent. As little as 1 U/ml of rIL4 significantly decreased both LAK activity and proliferation of cells induced by incubation of PBMC with 200 U/ml rIL2. Suppression was specific for the induction phase of LAK activity, because rIL4 did not suppress the cytotoxic capability of previously activated LAK cells. rIL4 directly suppressed rIL2-induced activation of precursors into LAK cells, since it inhibited both rIL2-induced proliferation and LAK cell activity in LGL-enriched cell fractions or in purified CD16+ NK cells. Suppressive effects of rIL4 were overcome by adding rIFNγ to the culture. These results suggest that rIL4 is a potent inhibitor of rIL2-induced activation of PBL, and that the net LAK response in the host may be a function of the production of IL-2, IL-4 and rIFNγ.

Original languageEnglish (US)
Pages (from-to)227-235
Number of pages9
JournalLymphokine Research
Volume7
Issue number3
StatePublished - 1988
Externally publishedYes

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Interleukin-4
Interleukin-2
Lymphokine-Activated Killer Cells
Lymphocytes
Lymphokines
Blood Cells
Natural Killer Cells
Cell Proliferation
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Recombinant interleukin 4 (rIL4) inhibits interleukin 2-induced activation of peripheral blood lymphocytes. / Han, X.; Itoh, K.; Balch, Charles M.; Pellis, N. R.

In: Lymphokine Research, Vol. 7, No. 3, 1988, p. 227-235.

Research output: Contribution to journalArticle

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AU - Balch, Charles M.

AU - Pellis, N. R.

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AB - Recombinant interleukin 4 (rIL4) inhibited the proliferation and induction of lymphokine activated killer (LAK) cells in human peripheral blood mononuclear cells (PBMC) cultured in recombinant interleukin 2 (rIL2). Other recombinant cytokines, such as rIFNγ, rTNFα, rIL1, and rIL3, did not inhibit LAK cell induction. rIL4-mediated suppression was dose dependent. As little as 1 U/ml of rIL4 significantly decreased both LAK activity and proliferation of cells induced by incubation of PBMC with 200 U/ml rIL2. Suppression was specific for the induction phase of LAK activity, because rIL4 did not suppress the cytotoxic capability of previously activated LAK cells. rIL4 directly suppressed rIL2-induced activation of precursors into LAK cells, since it inhibited both rIL2-induced proliferation and LAK cell activity in LGL-enriched cell fractions or in purified CD16+ NK cells. Suppressive effects of rIL4 were overcome by adding rIFNγ to the culture. These results suggest that rIL4 is a potent inhibitor of rIL2-induced activation of PBL, and that the net LAK response in the host may be a function of the production of IL-2, IL-4 and rIFNγ.

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