Recombinant human macrophage colony-stimulating factor augments pulmonary host defences against aspergillus fumigatus

Corina E. Gonzalez, Caron A. Lyman, Sondra Lee, Carla Del Guercio, Emmanuel Roilides, John Bacher, Andreas Gehrt, Erwin Feuerstein, Maria Tsokos, Thomas J. Walsh

Research output: Contribution to journalArticle

Abstract

The in vivo and ex vivo effects of macrophage colony-stimulating factor (M-CSF) were studied in a profoundly neutropenic rabbit model in order to determine its potential to augment pulmonary host defence against Aspergillus. M-CSF (100-600 μg/kg/d) was administered prophylactically to neutropenic rabbits with pulmonary aspergillosis starting three days pre-inoculation and then throughout neutropenia. Rabbits receiving M-CSF had significantly increased survival (P=0.01) and decreased pulmonary injury, as measured by decreased pulmonary infarction (P=0.004), when compared with untreated controls. Microscopic studies demonstrated greater numbers of activated pulmonary alveolar macrophages (PAMs) in lung tissue of rabbits receiving M-CSF, in comparison to controls (P<0.001). PAMs harvested from rabbits treated with M-CSF had a significantly greater percent phagocytosis of Aspergillus fumigatus conidia than did PAMs from controls (P=0.04). These data indicate that prophylactic administration of M-CSF augments pulmonary host defence against A. fumigatus and suggest a potential role for this cytokine as adjunctive therapy in the treatment of pulmonary aspergillosis in the setting of profound neutropenia.

Original languageEnglish (US)
Pages (from-to)87-95
Number of pages9
JournalCytokine
Volume15
Issue number2
DOIs
StatePublished - Jan 1 2001

Keywords

  • Aspergillus
  • Host defense
  • Macrophage-Colony-Stimulating factor
  • Pulmonary

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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    Gonzalez, C. E., Lyman, C. A., Lee, S., Del Guercio, C., Roilides, E., Bacher, J., Gehrt, A., Feuerstein, E., Tsokos, M., & Walsh, T. J. (2001). Recombinant human macrophage colony-stimulating factor augments pulmonary host defences against aspergillus fumigatus. Cytokine, 15(2), 87-95. https://doi.org/10.1006/cyto.2001.0889