Recombinant human IL-1α and -1β potentiate IgE-mediated histamine release from human basophils

W. A. Massey, T. C. Randall, A. Kagey-Sobotka, J. A. Warner, S. M. MacDonald, S. Gillis, A. C. Allison, L. M. Lichtenstein

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we have explored the relationship between interleukins and human basophil activation. Previous studies by ourselves and others have found that recombinant human (rh) IL-3 causes histamine release. The ability to release histamine has also been claimed for IL-1 but we cannot confirm this. In experiments with the basophils of 29 donors (excluding one D2O responder), histamine release with 100 ng/ml rhIL-1α was 1.3 ± 1% (SEM), whereas with rhIL-1β, it was 0.8 ± 1%. Both IL-1α and -1β were also used at concentrations of 0.01 to 1000 ng/ml without causing release. Neither increasing the Ca2+ concentration nor adding D2O or cytochalasin B caused IL-1α and -1β to become secretagogues. rhIL-1, however, did augment IgE-dependent histamine release. The enhancement was similar with both rhIL-1α and -1β, i.e. they were dose-dependent between 0.1 and 3 ng/ml and reached a plateau from 3 to 100 ng/ml. At submaximal histamine release (<10%), there was enhancement of three IgE-dependent secretagogues: 125% with goat anti-human IgE (n = 7), 215% with Ag E (n = 19), and 260% with a histamine releasing factor (n = 7). Non-IgE-dependent stimuli (formyl-methionine-leucine-phenylaline and the ionophore A23187, n = 10) were enhanced <5%. rhIL-1-enhancement persisted after cell washing (n = 10). rhIL-1 was active in preparations of 50 to 75% pure basophils in which mononuclear cells were reduced by >95% (n = 4), and mAbH34 to IL-1β blocked the enhancement caused by that molecule. We postulate that basophils have an IL-1 receptor which, when occupied, upregulates the response to IgE-related signals. Thus, this work characterizes a second interaction between interleukins and the cells central to the allergic response.

Original languageEnglish (US)
Pages (from-to)1875-1880
Number of pages6
JournalJournal of Immunology
Volume143
Issue number6
StatePublished - 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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