Recombinant human gapM1 expressed in Pichia pastoris and its anti-diabetic effect

Xiang Mei, Renqian Du, Xi Li, Haiyan Huang, Min Yu, Qiqun Tang

Research output: Contribution to journalArticle

Abstract

Adiponectin is an adipokine predominantly synthesized and secreted by adipocytes in the white adipose tissue, and it can lower the blood glucose level and increase free fatty acid oxidation. In the current study, we developed the globular domain of adiponectin (gapM1) to treat type II diabetes. In both flask and fermentor, we cultivated Pichia pastoris expressing recombinant gapM1 and established the purification procedure by using gel filtration and anion exchange chromatography. To evaluate the biological activity of recombinant gapM1, we used rat type II diabetes model fed high-fat diet in combination with low-dose STZ (Streptozocin) induction. We purified 200 mg gapM1 with purity of 96% from 10 liters of supernatant. The recombinant gapM1 significantly lowered blood glucose (34.2%), serum triglyceride (79.6%) and total cholesterol (62.1%) in type II diabetes induced rat. Therefore, the recombinant human gapM1 is successfully expressed in Pichia pastoris and effectively treated type II diabetes in rat models.

Original languageEnglish (US)
Pages (from-to)1211-1217
Number of pages7
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume25
Issue number8
StatePublished - 2009
Externally publishedYes

Keywords

  • GapM1
  • Pichia pastoris
  • Type II diabetes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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