Recombinant Histamine-Releasing Factor Enhances IgE-dependent IL-4 and IL-13 Secretion by Human Basophils

John T. Schroeder, Lawrence M. Lichtenstein, Susan M. MacDonald

Research output: Contribution to journalArticle

Abstract

Human recombinant histamine-releasing factor (HrHRF) is known to directly stimulate histamine release and IL-4 secretion from basophils of selected atopic donors In a reaction requiring the expression of a particular type of IgE, referred to as IgE+. In this study, HrHRF is shown to affect the IgE-mediated release of IL-4, IL-13, and histamine from basophils not normally releasing to this protein (i.e, those expressing IgE-). Priming with several different concentrations of HrHRF for 15 min enhanced basophil secretion of IL-4 and histamine after 4 h in a dose-dependent fashion following activation with anti-IgE Ab (10 ng/ml). This effect of HrHRF priming also occurred in cultures activated with 1 or 100 ng/ml of anti-IgE Ab. The secretion of IL-13 protein was enhanced similarly by HrHRF priming in cultures stimulated for 16 to 20 h with anti-IgE Ab. There were, however, no apparent changes In the secretion of histamine or cytokine by basophils primed with HrHRF and activated with the IgE-independent secretogogue, FMLP. These findings suggest that HrHRF modifies the response of basophils for IgE-dependent secretion by binding to a specific receptor, broadening the possible role of this protein in chronic allergic Inflammation.

Original languageEnglish (US)
Pages (from-to)447-452
Number of pages6
JournalJournal of Immunology
Volume159
Issue number1
StatePublished - Dec 1 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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