Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy

Paul F. Robbins, Mona El-Gamil, Yutaka Kawakami, Steven A. Rosenberg

Research output: Contribution to journalArticle

Abstract

The observation that allogeneic melanoma cells matched for particular HLA class I alleles stimulate T-cells isolated from patients suggest that widely shared antigens exist on these tumors. A transient expression system was developed for screening a melanoma complementary DNA library using the highly transfectable human kidney cell line 293. Using this system, large numbers of complementary DNA clones can be rapidly screened for the expression of antigens which stimulate T-cells. Tumor-infiltrating lymphocytes from patient 888, which recognized melanoma in the context of HLA-A24, were used to screen a complementary DNA library made from the autologous melanoma. Our results demonstrate that these tumor-infiltrating lymphocytes recognize tyrosinase, a gene previously shown to be recognized by T-cells only in the context of HLA- A2. These data demonstrate that a single antigen can be recognized in the context of two different class 1 HLA alleles. In addition, this study suggests that recognition of tyrosinase by antigen-specific T-cells may be involved in tumor rejection.

Original languageEnglish (US)
Pages (from-to)3124-3126
Number of pages3
JournalCancer Research
Volume54
Issue number12
StatePublished - Jun 15 1994
Externally publishedYes

Fingerprint

Tumor-Infiltrating Lymphocytes
Monophenol Monooxygenase
Immunotherapy
Melanoma
T-Lymphocytes
Antigens
Complementary DNA
Gene Library
HLA-A24 Antigen
Alleles
HLA-A2 Antigen
HEK293 Cells
Neoplasms
Clone Cells
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Robbins, P. F., El-Gamil, M., Kawakami, Y., & Rosenberg, S. A. (1994). Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy. Cancer Research, 54(12), 3124-3126.

Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy. / Robbins, Paul F.; El-Gamil, Mona; Kawakami, Yutaka; Rosenberg, Steven A.

In: Cancer Research, Vol. 54, No. 12, 15.06.1994, p. 3124-3126.

Research output: Contribution to journalArticle

Robbins, PF, El-Gamil, M, Kawakami, Y & Rosenberg, SA 1994, 'Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy', Cancer Research, vol. 54, no. 12, pp. 3124-3126.
Robbins PF, El-Gamil M, Kawakami Y, Rosenberg SA. Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy. Cancer Research. 1994 Jun 15;54(12):3124-3126.
Robbins, Paul F. ; El-Gamil, Mona ; Kawakami, Yutaka ; Rosenberg, Steven A. / Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy. In: Cancer Research. 1994 ; Vol. 54, No. 12. pp. 3124-3126.
@article{4e5def7a6ab44c9ba921278028484438,
title = "Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy",
abstract = "The observation that allogeneic melanoma cells matched for particular HLA class I alleles stimulate T-cells isolated from patients suggest that widely shared antigens exist on these tumors. A transient expression system was developed for screening a melanoma complementary DNA library using the highly transfectable human kidney cell line 293. Using this system, large numbers of complementary DNA clones can be rapidly screened for the expression of antigens which stimulate T-cells. Tumor-infiltrating lymphocytes from patient 888, which recognized melanoma in the context of HLA-A24, were used to screen a complementary DNA library made from the autologous melanoma. Our results demonstrate that these tumor-infiltrating lymphocytes recognize tyrosinase, a gene previously shown to be recognized by T-cells only in the context of HLA- A2. These data demonstrate that a single antigen can be recognized in the context of two different class 1 HLA alleles. In addition, this study suggests that recognition of tyrosinase by antigen-specific T-cells may be involved in tumor rejection.",
author = "Robbins, {Paul F.} and Mona El-Gamil and Yutaka Kawakami and Rosenberg, {Steven A.}",
year = "1994",
month = "6",
day = "15",
language = "English (US)",
volume = "54",
pages = "3124--3126",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "12",

}

TY - JOUR

T1 - Recognition of tyrosinase by tumor-infiltrating lymphocytes from a patient responding to immunotherapy

AU - Robbins, Paul F.

AU - El-Gamil, Mona

AU - Kawakami, Yutaka

AU - Rosenberg, Steven A.

PY - 1994/6/15

Y1 - 1994/6/15

N2 - The observation that allogeneic melanoma cells matched for particular HLA class I alleles stimulate T-cells isolated from patients suggest that widely shared antigens exist on these tumors. A transient expression system was developed for screening a melanoma complementary DNA library using the highly transfectable human kidney cell line 293. Using this system, large numbers of complementary DNA clones can be rapidly screened for the expression of antigens which stimulate T-cells. Tumor-infiltrating lymphocytes from patient 888, which recognized melanoma in the context of HLA-A24, were used to screen a complementary DNA library made from the autologous melanoma. Our results demonstrate that these tumor-infiltrating lymphocytes recognize tyrosinase, a gene previously shown to be recognized by T-cells only in the context of HLA- A2. These data demonstrate that a single antigen can be recognized in the context of two different class 1 HLA alleles. In addition, this study suggests that recognition of tyrosinase by antigen-specific T-cells may be involved in tumor rejection.

AB - The observation that allogeneic melanoma cells matched for particular HLA class I alleles stimulate T-cells isolated from patients suggest that widely shared antigens exist on these tumors. A transient expression system was developed for screening a melanoma complementary DNA library using the highly transfectable human kidney cell line 293. Using this system, large numbers of complementary DNA clones can be rapidly screened for the expression of antigens which stimulate T-cells. Tumor-infiltrating lymphocytes from patient 888, which recognized melanoma in the context of HLA-A24, were used to screen a complementary DNA library made from the autologous melanoma. Our results demonstrate that these tumor-infiltrating lymphocytes recognize tyrosinase, a gene previously shown to be recognized by T-cells only in the context of HLA- A2. These data demonstrate that a single antigen can be recognized in the context of two different class 1 HLA alleles. In addition, this study suggests that recognition of tyrosinase by antigen-specific T-cells may be involved in tumor rejection.

UR - http://www.scopus.com/inward/record.url?scp=0028363683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028363683&partnerID=8YFLogxK

M3 - Article

VL - 54

SP - 3124

EP - 3126

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 12

ER -