Abstract
We have found that the bacterial transposon Tn7 can recognize and preferentially insert adjacent to triple-helical nucleic acid structures. Both synthetic intermolecular triplexes, formed through the pairing of a short triplex-forming oligonucleotide on a plasmid DNA, and naturally occurring mirror repeat sequences known to form intramolecular triplexes or H-form DNA are preferential targets for Tn7 insertion in vitro. This target site selectivity depends upon the recognition of the triplex region by a Tn7- encoded ATP-using protein, TnsC, which controls Tn7 target site selection: the interaction of TnsC with the triplex region results in recruitment and activation of the Tn7 transposase. Recognition of a nucleic acid structural motif provides both new information into the factors that influence Tn7's target site selection and broadens its targeting capabilities.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3936-3941 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 97 |
| Issue number | 8 |
| DOIs | |
| State | Published - Apr 11 2000 |
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ASJC Scopus subject areas
- Genetics
- General
Cite this
Recognition of triple-helical DNA structures by transposon Tn7. / Rao, Jason E.; Miller, Paul S.; Craig, Nancy L.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 8, 11.04.2000, p. 3936-3941.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recognition of triple-helical DNA structures by transposon Tn7
AU - Rao, Jason E.
AU - Miller, Paul S.
AU - Craig, Nancy L
PY - 2000/4/11
Y1 - 2000/4/11
N2 - We have found that the bacterial transposon Tn7 can recognize and preferentially insert adjacent to triple-helical nucleic acid structures. Both synthetic intermolecular triplexes, formed through the pairing of a short triplex-forming oligonucleotide on a plasmid DNA, and naturally occurring mirror repeat sequences known to form intramolecular triplexes or H-form DNA are preferential targets for Tn7 insertion in vitro. This target site selectivity depends upon the recognition of the triplex region by a Tn7- encoded ATP-using protein, TnsC, which controls Tn7 target site selection: the interaction of TnsC with the triplex region results in recruitment and activation of the Tn7 transposase. Recognition of a nucleic acid structural motif provides both new information into the factors that influence Tn7's target site selection and broadens its targeting capabilities.
AB - We have found that the bacterial transposon Tn7 can recognize and preferentially insert adjacent to triple-helical nucleic acid structures. Both synthetic intermolecular triplexes, formed through the pairing of a short triplex-forming oligonucleotide on a plasmid DNA, and naturally occurring mirror repeat sequences known to form intramolecular triplexes or H-form DNA are preferential targets for Tn7 insertion in vitro. This target site selectivity depends upon the recognition of the triplex region by a Tn7- encoded ATP-using protein, TnsC, which controls Tn7 target site selection: the interaction of TnsC with the triplex region results in recruitment and activation of the Tn7 transposase. Recognition of a nucleic acid structural motif provides both new information into the factors that influence Tn7's target site selection and broadens its targeting capabilities.
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UR - http://www.scopus.com/inward/citedby.url?scp=0034636031&partnerID=8YFLogxK
U2 - 10.1073/pnas.080061497
DO - 10.1073/pnas.080061497
M3 - Article
C2 - 10737770
AN - SCOPUS:0034636031
VL - 97
SP - 3936
EP - 3941
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 8
ER -