Recognition of Dopamine Transporters by Antipeptide Antibodies

Roxanne A. Vaughan, George Uhl, Michael J. Kuhar

Research output: Contribution to journalArticle

Abstract

Hemocyanin-conjugated peptides corresponding to amino acid sequences from 14 different regions of the rat dopamine transporter were used to immunize rabbits and the resulting antisera were tested for dopamine transporter recognition. Eleven of the peptide sequences produced antipeptide antibodies detectable with ELISA assays. Sera directed against four of the peptides immunoprecipitated striatal transporters photoaffinity labeled with [125I]DEEP. Antisera directed against a peptide sequence at the C-terminal region of the transporter's putative second extracellular loop, "peptide 5," recognized photolabeled dopamine transporter protein from rat, dog, and human basal ganglia. These sera also recognized dopamine transporters from rat striatum in immunoblots; however, no reaction was found when rat cerebellum or frontal cortical tissue was used. These results provide further evidence that the cloned cDNA and its deduced amino acid sequence represents the protein expressed in brain and highlights the regions of the transporter which may be promising sites for the development of high-affinity antibodies.

Original languageEnglish (US)
Pages (from-to)209-215
Number of pages7
JournalMolecular and Cellular Neuroscience
Volume4
Issue number2
DOIs
StatePublished - Apr 1993
Externally publishedYes

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Dopamine Plasma Membrane Transport Proteins
Peptides
Antibodies
Immune Sera
Amino Acid Sequence
Corpus Striatum
Hemocyanin
Antibody Affinity
Basal Ganglia
Serum
Cerebellum
Complementary DNA
Enzyme-Linked Immunosorbent Assay
Dogs
Rabbits
Brain
Proteins

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience
  • Molecular Biology

Cite this

Recognition of Dopamine Transporters by Antipeptide Antibodies. / Vaughan, Roxanne A.; Uhl, George; Kuhar, Michael J.

In: Molecular and Cellular Neuroscience, Vol. 4, No. 2, 04.1993, p. 209-215.

Research output: Contribution to journalArticle

Vaughan, Roxanne A. ; Uhl, George ; Kuhar, Michael J. / Recognition of Dopamine Transporters by Antipeptide Antibodies. In: Molecular and Cellular Neuroscience. 1993 ; Vol. 4, No. 2. pp. 209-215.
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