Recognition of aminoacyl-tRNA: A common molecular mechanism revealed by cryo-EM

Wen Li, Xabier Agirrezabala, Jianlin Lei, Lamine Bouakaz, Julie L. Brunelle, Rodrigo F. Ortiz-Meoz, Rachel Green, Suparna Sanyal, Måns Ehrenberg, Joachim Frank

Research output: Contribution to journalArticle

Abstract

The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNAPhe, Trp-tRNATrp, or Leu-tRNALeuI. The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.

Original languageEnglish (US)
Pages (from-to)3322-3331
Number of pages10
JournalThe EMBO journal
Volume27
Issue number24
DOIs
StatePublished - Dec 17 2008

Fingerprint

Transfer RNA
Anticodon
Peptide Elongation Factor Tu
Ribosomes
RNA, Transfer, Phe
GTP Phosphohydrolases
Codon
Conformations
Proteins

Keywords

  • Cryo-EM
  • Decoding
  • EF-Tu
  • Ribosome

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Li, W., Agirrezabala, X., Lei, J., Bouakaz, L., Brunelle, J. L., Ortiz-Meoz, R. F., ... Frank, J. (2008). Recognition of aminoacyl-tRNA: A common molecular mechanism revealed by cryo-EM. The EMBO journal, 27(24), 3322-3331. https://doi.org/10.1038/emboj.2008.243

Recognition of aminoacyl-tRNA : A common molecular mechanism revealed by cryo-EM. / Li, Wen; Agirrezabala, Xabier; Lei, Jianlin; Bouakaz, Lamine; Brunelle, Julie L.; Ortiz-Meoz, Rodrigo F.; Green, Rachel; Sanyal, Suparna; Ehrenberg, Måns; Frank, Joachim.

In: The EMBO journal, Vol. 27, No. 24, 17.12.2008, p. 3322-3331.

Research output: Contribution to journalArticle

Li, W, Agirrezabala, X, Lei, J, Bouakaz, L, Brunelle, JL, Ortiz-Meoz, RF, Green, R, Sanyal, S, Ehrenberg, M & Frank, J 2008, 'Recognition of aminoacyl-tRNA: A common molecular mechanism revealed by cryo-EM', The EMBO journal, vol. 27, no. 24, pp. 3322-3331. https://doi.org/10.1038/emboj.2008.243
Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF et al. Recognition of aminoacyl-tRNA: A common molecular mechanism revealed by cryo-EM. The EMBO journal. 2008 Dec 17;27(24):3322-3331. https://doi.org/10.1038/emboj.2008.243
Li, Wen ; Agirrezabala, Xabier ; Lei, Jianlin ; Bouakaz, Lamine ; Brunelle, Julie L. ; Ortiz-Meoz, Rodrigo F. ; Green, Rachel ; Sanyal, Suparna ; Ehrenberg, Måns ; Frank, Joachim. / Recognition of aminoacyl-tRNA : A common molecular mechanism revealed by cryo-EM. In: The EMBO journal. 2008 ; Vol. 27, No. 24. pp. 3322-3331.
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