Receptor-specific induction of NF-κB components in primary B cells

Delicia A. Francis, Ranjan Sen, Nancy Rice, Thomas L. Rothstein

Research output: Contribution to journalArticle

Abstract

The NF-κB transcription factor complex plays a key role in the expression of genes involved in immune responses. Nuclear NF-κB is induced in B lymphocytes by engagement of either the antigen receptor (sIg) or the CD40 receptor for a T cell activation antigen, although different intracellular pathways appear to be involved. In the present study the protein composition of NF-κB complexes triggered by sIg and CD40 was probed by electrophoretic mobility shift, supershift, shift-Western, and Western blot analyses. At the time of peak NF-κB induction (2 h), the NF-κB components detected in the complexes induced through sIg and through CD40 were the same. However, with continued stimulation RelB completely disappeared from anti-Ig-stimulated κB binding material, but remained a component of CD40L-induced NF-κB. The loss of DNA-binding RelB from anti-Ig-induced NF-κB did not result from depletion of RelB from B cell nuclei, suggesting specific regulation of RelB function which is not directly attributed to IκB function. These results indicate that NF-κB complexes may undergo protein-specific alterations in a time- and receptor-dependent fashion that may be associated with differences in the outcomes of B cell stimulation through sIg and CD40.

Original languageEnglish (US)
Pages (from-to)285-293
Number of pages9
JournalInternational Immunology
Volume10
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • B cell antigen receptor
  • B lymphocytes
  • CD40 receptor
  • DNA-binding proteins
  • IκB proteins
  • RelB protein
  • Transcription factors

ASJC Scopus subject areas

  • Immunology

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