Receptor-mediated endocytosis in the bloodstream form of Trypanosoma brucei.

Isabelle Coppens, F. R. Opperdoes, P. J. Courtoy, P. Baudhuin

Research output: Contribution to journalArticle

Abstract

The uptake of various host plasma proteins by the bloodstream form of Trypanosoma brucei was studied both biochemically, using radiolabeled proteins, and with the electron microscope, using colloidal gold particles as molecular tracers onto which plasma proteins had been adsorbed. Total plasma proteins and serum albumin were taken up by a mechanism of fluid endocytosis with low clearance (0.1 microliter [mg cell protein]-1 h-1), while low-density lipoprotein (LDL) and transferrin were taken up by a receptor-mediated process with a clearance of two to three orders of magnitude higher than that of serum albumin. Binding prior to uptake of LDL and transferrin was saturable, depended on the presence of Ca2+, and the labeled ligand could be displaced by the homologous but not by heterologous protein. Binding of gold-labeled proteins was seen only to the membrane of the flagellar pocket and not elsewhere on the plasma membrane. After 1 h of incubation at 30 degrees C with gold-labeled LDL and transferrin, labeled cellular structures represented respectively half and one-third of the total volume of all single-membrane bounded endocytotic and electron-dense vacuoles within the cell.

Original languageEnglish (US)
Pages (from-to)465-473
Number of pages9
JournalJournal of Protozoology
Volume34
Issue number4
StatePublished - Nov 1987
Externally publishedYes

Fingerprint

Trypanosoma brucei brucei
Endocytosis
Transferrin
LDL Lipoproteins
Serum Albumin
Blood Proteins
Gold
Proteins
Electrons
Gold Colloid
Membranes
Cellular Structures
Vacuoles
Cell Membrane
Ligands

ASJC Scopus subject areas

  • Parasitology

Cite this

Coppens, I., Opperdoes, F. R., Courtoy, P. J., & Baudhuin, P. (1987). Receptor-mediated endocytosis in the bloodstream form of Trypanosoma brucei. Journal of Protozoology, 34(4), 465-473.

Receptor-mediated endocytosis in the bloodstream form of Trypanosoma brucei. / Coppens, Isabelle; Opperdoes, F. R.; Courtoy, P. J.; Baudhuin, P.

In: Journal of Protozoology, Vol. 34, No. 4, 11.1987, p. 465-473.

Research output: Contribution to journalArticle

Coppens, I, Opperdoes, FR, Courtoy, PJ & Baudhuin, P 1987, 'Receptor-mediated endocytosis in the bloodstream form of Trypanosoma brucei.', Journal of Protozoology, vol. 34, no. 4, pp. 465-473.
Coppens, Isabelle ; Opperdoes, F. R. ; Courtoy, P. J. ; Baudhuin, P. / Receptor-mediated endocytosis in the bloodstream form of Trypanosoma brucei. In: Journal of Protozoology. 1987 ; Vol. 34, No. 4. pp. 465-473.
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