Recent insights on RNA folding mechanisms from catalytic RNA

S. A. Woodson

Research output: Contribution to journalArticle

Abstract

Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly. These kinetically trapped intermediates arise from alternative secondary structures that form early in the folding process. In cells, proteins modulate the outcome of RNA folding reactions by stabilizing specific conformations or by accelerating refolding of misfolded intermediates. At the same time, competition between metastable conformations provides a means for regulating the biological activity of transcripts.

Original languageEnglish (US)
Pages (from-to)796-808
Number of pages13
JournalCellular and Molecular Life Sciences
Volume57
Issue number5
DOIs
StatePublished - 2000

Fingerprint

RNA Folding
Catalytic RNA
Conformations
RNA
Bioactivity
Proteins
In Vitro Techniques

Keywords

  • Energy landscape
  • Folding kinetics
  • Group I intron
  • Ribozyme
  • RNA folding
  • RNA structure
  • RNase P
  • Transcription elongation
  • Translation attenuation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

Cite this

Recent insights on RNA folding mechanisms from catalytic RNA. / Woodson, S. A.

In: Cellular and Molecular Life Sciences, Vol. 57, No. 5, 2000, p. 796-808.

Research output: Contribution to journalArticle

@article{e1e0666f866041e285bbcd02548297e5,
title = "Recent insights on RNA folding mechanisms from catalytic RNA",
abstract = "Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly. These kinetically trapped intermediates arise from alternative secondary structures that form early in the folding process. In cells, proteins modulate the outcome of RNA folding reactions by stabilizing specific conformations or by accelerating refolding of misfolded intermediates. At the same time, competition between metastable conformations provides a means for regulating the biological activity of transcripts.",
keywords = "Energy landscape, Folding kinetics, Group I intron, Ribozyme, RNA folding, RNA structure, RNase P, Transcription elongation, Translation attenuation",
author = "Woodson, {S. A.}",
year = "2000",
doi = "10.1007/s000180050042",
language = "English (US)",
volume = "57",
pages = "796--808",
journal = "Cellular and Molecular Life Sciences",
issn = "1420-682X",
publisher = "Birkhauser Verlag Basel",
number = "5",

}

TY - JOUR

T1 - Recent insights on RNA folding mechanisms from catalytic RNA

AU - Woodson, S. A.

PY - 2000

Y1 - 2000

N2 - Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly. These kinetically trapped intermediates arise from alternative secondary structures that form early in the folding process. In cells, proteins modulate the outcome of RNA folding reactions by stabilizing specific conformations or by accelerating refolding of misfolded intermediates. At the same time, competition between metastable conformations provides a means for regulating the biological activity of transcripts.

AB - Methods for probing RNA structure in real time have revealed that initial folding steps are complete in less than a second. Refolding of large catalytic RNAs in vitro often results in long-lived intermediates that reach the native structure very slowly. These kinetically trapped intermediates arise from alternative secondary structures that form early in the folding process. In cells, proteins modulate the outcome of RNA folding reactions by stabilizing specific conformations or by accelerating refolding of misfolded intermediates. At the same time, competition between metastable conformations provides a means for regulating the biological activity of transcripts.

KW - Energy landscape

KW - Folding kinetics

KW - Group I intron

KW - Ribozyme

KW - RNA folding

KW - RNA structure

KW - RNase P

KW - Transcription elongation

KW - Translation attenuation

UR - http://www.scopus.com/inward/record.url?scp=0034043484&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034043484&partnerID=8YFLogxK

U2 - 10.1007/s000180050042

DO - 10.1007/s000180050042

M3 - Article

VL - 57

SP - 796

EP - 808

JO - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

SN - 1420-682X

IS - 5

ER -