Recent developments in the use of γ-H2AX as a quantitative DNA double-strand break biomarker

Christophe E. Redon, Asako J. Nakamura, Olga A. Martin, Palak R. Parekh, Urbain S. Weyemi, William M. Bonner

Research output: Contribution to journalArticlepeer-review

Abstract

The past year has seen considerable developments in the use of the DNA double-strand breaks (DSBs) to evaluate genome alterations in cells undergoing a variety of genotoxic stresses in vitro and in vivo. When the γ-H2AX foci which mark the DSBs are stained, individual breaks are detectible, making the assay suitable for situations requiring great sensitivity. While the methods for the detection of γ-H2AX foci are still evolving, particularly for in vivo detection, the basic assay has proven to be useful in several diverse areas of research. We will highlight recent developments of the assay in four areas: radiation biodosimetry, the evaluation or validation of new cancer drugs in clinical studies, chronic inflammation, and environmental genotoxicity.

Original languageEnglish (US)
Pages (from-to)168-174
Number of pages7
JournalAging
Volume3
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Keywords

  • Biomarker
  • Cancer
  • DNA damage
  • DNA repair
  • Double-strand break
  • Senescent cells
  • γ-H2AX

ASJC Scopus subject areas

  • Aging
  • Cell Biology

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