Recent advances in micro-scale and nano-scale high-performance liquid-phase chromatography for proteome research

Dingyin Tao, Lihua Zhang, Yichu Shan, Zhen Liang, Yukui Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS-MS) is regarded as one of the most powerful techniques for separation and identification of proteins. Recently, much effort has been made to improve the separation capacity, detection sensitivity, and analysis throughput of micro- and nano-HPLC, by increasing column length, reducing column internal diameter, and using integrated techniques. Development of HPLC columns has also been rapid, as a result of the use of submicrometer packing materials and monolithic columns. All these innovations result in clearly improved performance of micro- and nano-HPLC for proteome research.

Original languageEnglish (US)
Pages (from-to)229-241
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
Volume399
Issue number1
DOIs
StatePublished - Jan 2011

Keywords

  • MS-MS
  • Micro-HPLC
  • Multi-dimensional separation
  • Nano-HPLC
  • Proteome

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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