The helper T cell clone 3H.25 is specific for hen egg white lysozyme and the class II MHC molecule I-Ab. This TH cell has three rearrangements in the β-chain gene family-a Vβ-Dβ-Jpβ1 and a Dβ2-Jβ2 rearrangement on one homolog and a Dβ1-Jβ2 rearrangement on the other. These observations demonstrate that this functional T lymphocyte expresses only a single Vβ gene segment and, accordingly, exhibits allelic exclusion of β-chain gene expression. The rearranged 3H.25 Vβ gene segment is the same as that expressed in a T helper cell specific for cytochrome c and an I-Ek MHC molecule. Thus, there is no simple correlation between the Vβ gene segment and antigen specificity or MHC restriction.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)