Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme: No correlation between Vβ and MHC restriction

Joan Goverman; Karyl Minard; Nilabh Shastri; Tim Hunkapiller; Dan Hansburg; Eli Sercarz; Leroy Hood

doi:10.1016/0092-8674(85)90345-9

Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme: No correlation between V_β and MHC restriction

Joan Goverman, Karyl Minard, Nilabh Shastri, Tim Hunkapiller, Dan Hansburg, Eli Sercarz, Leroy Hood

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

The helper T cell clone 3H.25 is specific for hen egg white lysozyme and the class II MHC molecule I-A^b. This T_H cell has three rearrangements in the β-chain gene family-a V_β-D_β-Jp_β1 and a D_β2-J_β2 rearrangement on one homolog and a D_β1-J_β2 rearrangement on the other. These observations demonstrate that this functional T lymphocyte expresses only a single V_β gene segment and, accordingly, exhibits allelic exclusion of β-chain gene expression. The rearranged 3H.25 V_β gene segment is the same as that expressed in a T helper cell specific for cytochrome c and an I-E^k MHC molecule. Thus, there is no simple correlation between the V_β gene segment and antigen specificity or MHC restriction.

Original language	English (US)
Pages (from-to)	859-867
Number of pages	9
Journal	Cell
Volume	40
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(85)90345-9
State	Published - Apr 1985
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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@article{587765e696aa46e3ab3539afa15b142b,

title = "Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme: No correlation between Vβ and MHC restriction",

abstract = "The helper T cell clone 3H.25 is specific for hen egg white lysozyme and the class II MHC molecule I-Ab. This TH cell has three rearrangements in the β-chain gene family-a Vβ-Dβ-Jpβ1 and a Dβ2-Jβ2 rearrangement on one homolog and a Dβ1-Jβ2 rearrangement on the other. These observations demonstrate that this functional T lymphocyte expresses only a single Vβ gene segment and, accordingly, exhibits allelic exclusion of β-chain gene expression. The rearranged 3H.25 Vβ gene segment is the same as that expressed in a T helper cell specific for cytochrome c and an I-Ek MHC molecule. Thus, there is no simple correlation between the Vβ gene segment and antigen specificity or MHC restriction.",

author = "Joan Goverman and Karyl Minard and Nilabh Shastri and Tim Hunkapiller and Dan Hansburg and Eli Sercarz and Leroy Hood",

note = "Funding Information: The authors thank Dr. Suzanna Horvath and Marilyn Tomick for providing synthetic oligonucleotides. and Scheherazade Nasseri for making available lysozymsspecific bulk T cell lines from BALB.B mice, and Mr. Gerald Siu and Drs. Mitchell Kronenberg and Richard Barth for critical reading of the manuscript. We also want to thank Debbie Maloney for technical assistance and Connie Katz, Gwen Anastasi, and Bertha Jones for help in preparing the manuscript. J. G. is supported by a Leukemia Society of America Senior Fellowship. This work was supported in part by T Cell Sciences, Inc.",

year = "1985",

month = apr,

doi = "10.1016/0092-8674(85)90345-9",

language = "English (US)",

volume = "40",

pages = "859--867",

journal = "Cell",

issn = "0092-8674",

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T1 - Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme

T2 - No correlation between Vβ and MHC restriction

AU - Goverman, Joan

AU - Minard, Karyl

AU - Shastri, Nilabh

AU - Hunkapiller, Tim

AU - Hansburg, Dan

AU - Sercarz, Eli

AU - Hood, Leroy

N1 - Funding Information: The authors thank Dr. Suzanna Horvath and Marilyn Tomick for providing synthetic oligonucleotides. and Scheherazade Nasseri for making available lysozymsspecific bulk T cell lines from BALB.B mice, and Mr. Gerald Siu and Drs. Mitchell Kronenberg and Richard Barth for critical reading of the manuscript. We also want to thank Debbie Maloney for technical assistance and Connie Katz, Gwen Anastasi, and Bertha Jones for help in preparing the manuscript. J. G. is supported by a Leukemia Society of America Senior Fellowship. This work was supported in part by T Cell Sciences, Inc.

PY - 1985/4

Y1 - 1985/4

N2 - The helper T cell clone 3H.25 is specific for hen egg white lysozyme and the class II MHC molecule I-Ab. This TH cell has three rearrangements in the β-chain gene family-a Vβ-Dβ-Jpβ1 and a Dβ2-Jβ2 rearrangement on one homolog and a Dβ1-Jβ2 rearrangement on the other. These observations demonstrate that this functional T lymphocyte expresses only a single Vβ gene segment and, accordingly, exhibits allelic exclusion of β-chain gene expression. The rearranged 3H.25 Vβ gene segment is the same as that expressed in a T helper cell specific for cytochrome c and an I-Ek MHC molecule. Thus, there is no simple correlation between the Vβ gene segment and antigen specificity or MHC restriction.

AB - The helper T cell clone 3H.25 is specific for hen egg white lysozyme and the class II MHC molecule I-Ab. This TH cell has three rearrangements in the β-chain gene family-a Vβ-Dβ-Jpβ1 and a Dβ2-Jβ2 rearrangement on one homolog and a Dβ1-Jβ2 rearrangement on the other. These observations demonstrate that this functional T lymphocyte expresses only a single Vβ gene segment and, accordingly, exhibits allelic exclusion of β-chain gene expression. The rearranged 3H.25 Vβ gene segment is the same as that expressed in a T helper cell specific for cytochrome c and an I-Ek MHC molecule. Thus, there is no simple correlation between the Vβ gene segment and antigen specificity or MHC restriction.

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Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme: No correlation between V_β and MHC restriction

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