@article{38ea00a8caff469eb5183c2ce4a90ccb,
title = "Real-time quantitative PCR and droplet digital PCR for plant miRNAs in mammalian blood provide little evidence for general uptake of dietary miRNAs: Limited evidence for general uptake of dietary plant xenomiRs",
abstract = "Evidence that exogenous dietary miRNAs enter the bloodstream and tissues of ingesting animals has been accompanied by an indication that at least one plant miRNA, miR168, participates in {"}cross-kingdom{"} regulation of a mammalian transcript. If confirmed, these findings would support investigation of miRNA-based dietary interventions in disease. Here, blood was obtained pre- And post-prandially (1, 4, 12 h) from pigtailed macaques that received a miRNA-rich plantbased substance. Plant and endogenous miRNAs were measured by RT-qPCR. Although low-level amplification was observed for some plant miRNA assays, amplification was variable and possibly non-specific, as suggested by droplet digital PCR. A consistent response to dietary intake was not observed. While our results do not support general and consistent uptake of dietary plant miRNAs, additional studies are needed to establish whether or not plant or animal xenomiRs are transferred across the gut in sufficient quantity to regulate endogenous genes.",
keywords = "Diet, Digital PCR, Exosome, Extracellular vesicle, MicroRNA, Nutrition, Plant, RT-qPCR, Xenomir",
author = "Witwer, {Kenneth W.} and Mcalexander, {Melissa A.} and Queen, {Suzanne E.} and Adams, {Robert J.}",
note = "Funding Information: The authors thank members of the Department of Molecular and Comparative Pathobiology for interesting discussions and gratefully acknowledge the support of Janice E. Clements, M. Christine Zink and Joseph L. Mankowski, the assistance of Kelly A. Metcalf Pate, and the technical contributions of Sarah S. Subaran. KWW would like to thank Jasper D. Rine for insightful comments during a poster session at the 2013 Keystone Symposium “Nutrition, Epigenetics, and Human Disease,” at which a portion of this work was presented. The authors also thank the anonymous reviewers for their expert guidance. This project was supported by the National Center for Research Resources and the Office of Research Infrastructure Programs (ORIP) and the National Institutes of Health through Grant Number P40 OD013117. KWW and MAM are supported in part by NIMH Center grant P30 MHO75673, and K.W.W. receives support from NIH grants NS076357, MH070306 and AI096113 (J.E. Clements, PI).",
year = "2013",
month = jul,
doi = "10.4161/rna.25246",
language = "English (US)",
volume = "10",
pages = "1080--1086",
journal = "RNA Biology",
issn = "1547-6286",
publisher = "Landes Bioscience",
number = "7",
}