Real-Time Monitoring of a Protein Biomarker

Claudio Parolo, Andrea Idili, Gabriel Ortega, Andrew Csordas, Alex Hsu, Netzahualcoýotl Arroyo-Currás, Qin Yang, Brian Scott Ferguson, Jinpeng Wang, Kevin W. Plaxco

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to monitor protein biomarkers continuously and in real-time would significantly advance the precision of medicine. Current protein-detection techniques, however, including ELISA and lateral flow assays, provide only time-delayed, single-time-point measurements, limiting their ability to guide prompt responses to rapidly evolving, life-threatening conditions. In response, here we present an electrochemical aptamer-based sensor (EAB) that supports high-frequency, real-time biomarker measurements. Specifically, we have developed an electrochemical, aptamer-based (EAB) sensor against Neutrophil Gelatinase-Associated Lipocalin (NGAL), a protein that, if present in urine at levels above a threshold value, is indicative of acute renal/kidney injury (AKI). When deployed inside a urinary catheter, the resulting reagentless, wash-free sensor supports real-time, high-frequency monitoring of clinically relevant NGAL concentrations over the course of hours. By providing an "early warning system", the ability to measure levels of diagnostically relevant proteins such as NGAL in real-time could fundamentally change how we detect, monitor, and treat many important diseases.

Original languageEnglish (US)
Pages (from-to)1877-1881
Number of pages5
JournalACS sensors
Volume5
Issue number7
DOIs
StatePublished - Jul 24 2020

Keywords

  • acute kidney injury
  • aptamer
  • electrochemical sensor
  • neutrophil gelatinase-associated lipocalin
  • real-time protein monitoring

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

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