Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method

Toru Komatsu, Kai Johnsson, Hiroyuki Okuno, Haruhiko Bito, Takanari Inoue, Tetsuo Nagano, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

102 Scopus citations

Abstract

We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time imaging of protein labeling without any washout process and is uniquely suitable for real-time imaging of protein dynamics on the cell surface. We applied this method to address the spatiotemporal dynamics of the EGF receptor during cell migration.

Original languageEnglish (US)
Pages (from-to)6745-6751
Number of pages7
JournalJournal of the American Chemical Society
Volume133
Issue number17
DOIs
StatePublished - May 4 2011

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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