Real-time measurement of small molecules directly in awake, ambulatory animals

Netzahualcóyotl Arroyo-Currás, Jacob Somerson, Philip A. Vieira, Kyle L. Ploense, Tod E. Kippin, Kevin W. Plaxco

Research output: Contribution to journalArticle

Abstract

The development of a technology capable of tracking the levels of drugs, metabolites, and biomarkers in the body continuously and in real time would advance our understanding of health and our ability to detect and treat disease. It would, for example, enable therapies guided by high-resolution, patient-specific pharmacokinetics (including feedback-controlled drug delivery), opening new dimensions in personalized medicine. In response, we demonstrate here the ability of electrochemical aptamer-based (E-AB) sensors to support continuous, real-time, multihour measurements when emplaced directly in the circulatory systems of living animals. Specifically, we have used E-AB sensors to perform the multihour, real-time measurement of four drugs in the bloodstream of even awake, ambulatory rats, achieving precise molecular measurements at clinically relevant detection limits and high (3 s) temporal resolution, attributes suggesting that the approach could provide an important window into the study of physiology and pharmacokinetics.

Original languageEnglish (US)
Pages (from-to)645-650
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number4
DOIs
StatePublished - Jan 24 2017

Keywords

  • Aptamer
  • E-DNA
  • In vivo
  • Precision medicine
  • Square-wave voltammetry

ASJC Scopus subject areas

  • General

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