TY - JOUR
T1 - Real-Time Imaging Reveals Properties of Glutamate-Induced Arc/Arg 3.1 Translation in Neuronal Dendrites
AU - Na, Youn
AU - Park, Sungjin
AU - Lee, Changhee
AU - Kim, Dong Kyu
AU - Park, Joo Min
AU - Sockanathan, Shanthini
AU - Huganir, Richard L.
AU - Worley, Paul F.
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/8/3
Y1 - 2016/8/3
N2 - The immediate early gene Arc (also Arg3.1) produces rapid changes in synaptic properties that are linked to de novo translation. Here we develop a novel translation reporter that exploits the rapid maturation and “flash” kinetics of Gaussia luciferase (Gluc) to visualize Arc translation. Following glutamate stimulation, discrete Arc-Gluc bioluminescent flashes representing sites of de novo translation are detected within 15 s at distributed sites in dendrites, but not spines. Flashes are episodic, lasting ∼20 s, and may be unitary or repeated at ∼minute intervals at the same sites. Analysis of flash amplitudes suggests they represent the quantal product of one or more polyribosomes, while inter-flash intervals appear random, suggesting they arise from a stochastic process. Surprisingly, glutamate-induced translation is dependent on Arc open reading frame. Combined observations support a model in which stalled ribosomes are reactivated to rapidly generate Arc protein.
AB - The immediate early gene Arc (also Arg3.1) produces rapid changes in synaptic properties that are linked to de novo translation. Here we develop a novel translation reporter that exploits the rapid maturation and “flash” kinetics of Gaussia luciferase (Gluc) to visualize Arc translation. Following glutamate stimulation, discrete Arc-Gluc bioluminescent flashes representing sites of de novo translation are detected within 15 s at distributed sites in dendrites, but not spines. Flashes are episodic, lasting ∼20 s, and may be unitary or repeated at ∼minute intervals at the same sites. Analysis of flash amplitudes suggests they represent the quantal product of one or more polyribosomes, while inter-flash intervals appear random, suggesting they arise from a stochastic process. Surprisingly, glutamate-induced translation is dependent on Arc open reading frame. Combined observations support a model in which stalled ribosomes are reactivated to rapidly generate Arc protein.
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U2 - 10.1016/j.neuron.2016.06.017
DO - 10.1016/j.neuron.2016.06.017
M3 - Article
C2 - 27397520
AN - SCOPUS:84991060606
SN - 0896-6273
VL - 91
SP - 561
EP - 573
JO - Neuron
JF - Neuron
IS - 3
ER -