TY - GEN
T1 - Real-time imaging for cerebral ischemia in rats using the multi-wavelength handheld photoacoustic system
AU - Liu, Yu Hang
AU - Xu, Yu
AU - Chan, Kim Chuan
AU - Mehta, Kalpesh
AU - Thakor, Nitish
AU - Liao, Lun De
N1 - Publisher Copyright:
© 2017 SPIE.
PY - 2017
Y1 - 2017
N2 - Stroke is the second leading cause of death worldwide. Rapid and precise diagnosis is essential to expedite clinical decision and improve functional outcomes in stroke patients; therefore, real-time imaging plays an important role to provide crucial information for post-stroke recovery analysis. In this study, based on the multi-wavelength laser and 18.5 MHz array-based ultrasound platform, a real-time handheld photoacoustic (PA) system was developed to evaluate cerebrovascular functions pre- and post-stroke in rats. Using this system, hemodynamic information such as cerebral blood volume (CBV) can be acquired for assessment. One rat stroke model (i.e., photothrombotic ischemia (PTI)) was employed for evaluating the effect of local ischemia. For achieving better intrinsic PA contrast, Vantage and COMSOL simulations were applied to optimize the light delivery (e.g., interval between two arms) from customized fiber bundle, while phantom experiment was conducted to evaluate the imaging performance of this system. Results of phantom experiment showed that hairs (∼150 μm diameter) and pencil lead (500 μm diameter) can be imaged clearly. On the other hand, results of in vivo experiments also demonstrated that stroke symptoms can be observed in PTI model poststroke. In the near future, with the help of PA specific contrast agent, the system would be able to achieve blood-brain barrier leakage imaging post-stroke. Overall, the real-time handheld PA system holds great potential in disease models involving impairments in cerebrovascular functions.
AB - Stroke is the second leading cause of death worldwide. Rapid and precise diagnosis is essential to expedite clinical decision and improve functional outcomes in stroke patients; therefore, real-time imaging plays an important role to provide crucial information for post-stroke recovery analysis. In this study, based on the multi-wavelength laser and 18.5 MHz array-based ultrasound platform, a real-time handheld photoacoustic (PA) system was developed to evaluate cerebrovascular functions pre- and post-stroke in rats. Using this system, hemodynamic information such as cerebral blood volume (CBV) can be acquired for assessment. One rat stroke model (i.e., photothrombotic ischemia (PTI)) was employed for evaluating the effect of local ischemia. For achieving better intrinsic PA contrast, Vantage and COMSOL simulations were applied to optimize the light delivery (e.g., interval between two arms) from customized fiber bundle, while phantom experiment was conducted to evaluate the imaging performance of this system. Results of phantom experiment showed that hairs (∼150 μm diameter) and pencil lead (500 μm diameter) can be imaged clearly. On the other hand, results of in vivo experiments also demonstrated that stroke symptoms can be observed in PTI model poststroke. In the near future, with the help of PA specific contrast agent, the system would be able to achieve blood-brain barrier leakage imaging post-stroke. Overall, the real-time handheld PA system holds great potential in disease models involving impairments in cerebrovascular functions.
KW - Cerebral blood volume
KW - Hemodynamic function
KW - Photoacoustic imaging
KW - Photothrombotic ischemia
KW - Real-time handheld PA imaging system
UR - http://www.scopus.com/inward/record.url?scp=85019170845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019170845&partnerID=8YFLogxK
U2 - 10.1117/12.2251775
DO - 10.1117/12.2251775
M3 - Conference contribution
AN - SCOPUS:85019170845
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Neural Imaging and Sensing
A2 - Ding, Jun
A2 - Luo, Qingming
PB - SPIE
T2 - Neural Imaging and Sensing
Y2 - 30 January 2017 through 31 January 2017
ER -