Real-time imaging for cerebral ischemia in rats using the multi-wavelength handheld photoacoustic system

Yu Hang Liu, Yu Xu, Kim Chuan Chan, Kalpesh Mehta, Nitish Thakor, Lun De Liao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Stroke is the second leading cause of death worldwide. Rapid and precise diagnosis is essential to expedite clinical decision and improve functional outcomes in stroke patients; therefore, real-time imaging plays an important role to provide crucial information for post-stroke recovery analysis. In this study, based on the multi-wavelength laser and 18.5 MHz array-based ultrasound platform, a real-time handheld photoacoustic (PA) system was developed to evaluate cerebrovascular functions pre- and post-stroke in rats. Using this system, hemodynamic information such as cerebral blood volume (CBV) can be acquired for assessment. One rat stroke model (i.e., photothrombotic ischemia (PTI)) was employed for evaluating the effect of local ischemia. For achieving better intrinsic PA contrast, Vantage and COMSOL simulations were applied to optimize the light delivery (e.g., interval between two arms) from customized fiber bundle, while phantom experiment was conducted to evaluate the imaging performance of this system. Results of phantom experiment showed that hairs (∼150 μm diameter) and pencil lead (500 μm diameter) can be imaged clearly. On the other hand, results of in vivo experiments also demonstrated that stroke symptoms can be observed in PTI model poststroke. In the near future, with the help of PA specific contrast agent, the system would be able to achieve blood-brain barrier leakage imaging post-stroke. Overall, the real-time handheld PA system holds great potential in disease models involving impairments in cerebrovascular functions.

Original languageEnglish (US)
Title of host publicationNeural Imaging and Sensing
EditorsJun Ding, Qingming Luo
ISBN (Electronic)9781510605435
StatePublished - 2017
EventNeural Imaging and Sensing - San Francisco, United States
Duration: Jan 30 2017Jan 31 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherNeural Imaging and Sensing
Country/TerritoryUnited States
CitySan Francisco


  • Cerebral blood volume
  • Hemodynamic function
  • Photoacoustic imaging
  • Photothrombotic ischemia
  • Real-time handheld PA imaging system

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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