Real-time dual MRI for predicting and subsequent validation of intra-arterial stem cell delivery to the central nervous system

Piotr Walczak, Miroslaw Janowski

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Stem cell therapy for neurological disorders reached a pivotal point when the efficacy of several cell types was demonstrated in small-animal models. Translation of stem cell therapy is contingent upon overcoming the challenge of effective cell delivery to the human brain, which has a volume of ~1000 times larger than that of the mouse. Intra-arterial (IA) injection can achieve a broad, global, but if needed also spatially targeted biodistribution; however, its utility has been limited by unpredictable cell destination and homing as dictated by the vascular territory, as well as by safety concerns. We show here that high-speed MRI can be used to visualize the intravascular distribution of a superparamagnetic iron oxide contrast agent and can thus be used to accurately predict the distribution of IA administered stem cells. Moreover, high-speed MRI enables the real-time visualization of cell homing, providing the opportunity for immediate intervention in the case of undesired biodistribution.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages175-191
Number of pages17
Volume126
DOIs
StatePublished - 2017

Publication series

NameNeuromethods
Volume126
ISSN (Print)08932336
ISSN (Electronic)19406045

Keywords

  • Intra-arterial
  • Iron oxide
  • MRI
  • Real-time
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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