Real-time detection of neurite outgrowth using microfluidic device

Samhwan Kim, Jongmoon Jang, Hongsoo Choi, Cheil Moon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a simple method for real-time detection of the neurite outgrowth using microfluidic device. Our microfluidic device contains three compartmentalized channels which are for cell seeding, hydrogel and growth factors. Collagen gel is filled in the middle channel and pheochromocytoma (PC12) cells are seeded in the left channel. To induce differentiation of PC12 cells, 50 ng/ml to1000 ng/ml of nerve growth factor (NGF) is introduced into the right channel. After three days of NGF treatment, PC12 cells begin to extend neurites and formed neurite network from sixth day. Quantification of neurite outgrowth is analyzed by measuring the total area of neurites. On sixth day, the area is doubled compared to the area on third day and increases by 20 times on ninth day.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume8879
DOIs
Publication statusPublished - 2013
Externally publishedYes
EventSPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju, Korea, Republic of
Duration: Feb 20 2013Feb 23 2013

Other

OtherSPIE Nano-Bio Sensing, Imaging, and Spectroscopy
CountryKorea, Republic of
CityJeju
Period2/20/132/23/13

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Keywords

  • Microfluidic device
  • Nerve growth factor
  • Neurite outgrowth

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Kim, S., Jang, J., Choi, H., & Moon, C. (2013). Real-time detection of neurite outgrowth using microfluidic device. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 8879). [887906] https://doi.org/10.1117/12.2018667