Abstract
Background: An acoustic wave immunosensor was developed to illustrate the viability of such devices in early detection of molecular cancer biomarkers. The methods described here involve a real-time, less invasive technique for detecting mesothelin, a protein that has been linked to pancreatic and ovarian cancer. Methods: Antibodies were immobilized on the gold surface of the device via a self-assembled alkanethiol monolayer. Supernatant from two different pancreatic cancer cell-lines (PL1 and CAPAN2) containing an unknown concentration of mesothelin was tested for the protein by a flow-through analytical technique in three types of experiments. Binding of the mesothelin to the immobilized antibody layer caused a shift in the device's resonant frequency, which was correlated to the concentration of supernatant. A reference sensor was used to correct for frequency shifts caused by pressure or viscosity effects from the injection of the supernatant solution. Results: Repeated experiments indicate that the sensors are capable of nanogram detection thresholds of mesothelin proteins at room temperature and in complex mixture. Conclusions: Acoustic wave device biosensors have the potential to become a valuable tool in screening for pancreatic as well as other types of cancers. The main features include real-time detection, high sensitivity, and ease of use.
Original language | English (US) |
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Pages (from-to) | 180-187 |
Number of pages | 8 |
Journal | Cancer detection and prevention |
Volume | 30 |
Issue number | 2 |
DOIs | |
State | Published - 2006 |
Keywords
- Antibody immobilization
- Biosensor
- Cancer biomarker detection
- Mesothelin
- Oscillator circuits
- Pancreatic cancer
- Piranha solution
- QCM-based immunosensors
- Quartz crystal microbalance
- Reference sensor
- Sauerbrey equation
- Screening patients
- Uranine soluton
ASJC Scopus subject areas
- Oncology
- Cancer Research