Real-time crystallographic studies of the adenine riboswitch using an X-ray free-electron laser

Jason R. Stagno, Yuba R. Bhandari, Chelsie E. Conrad, Yu Liu, Yun Xing Wang

Research output: Contribution to journalReview articlepeer-review

Abstract

Structures of the four reaction states of the adenine riboswitch aptamer domain, including a transient intermediate state were solved by serial femtosecond crystallography. The structures not only demonstrate the use of X-ray free-electron lasers for RNA crystallography but have also proven that transient states can be determined in real time by mix-and-inject crystallography. These results illustrate the structural basis for the ligand-induced conformational changes associated with the molecular ‘switch’.

Original languageEnglish (US)
Pages (from-to)3374-3380
Number of pages7
JournalFEBS Journal
Volume284
Issue number20
DOIs
StatePublished - Oct 2017
Externally publishedYes

Keywords

  • RNA
  • X-ray free-electron laser
  • adenine riboswitch
  • diffusive mixing
  • lattice conversion
  • phase transition
  • reaction intermediate
  • serial femtosecond crystallography
  • time-resolved crystallography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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