Real-time correlation of intracellular gene vector transport rates with biological location in live mesenchymal stem cells

J. Suh, Y. An, B. Tang, J. S. Suk, J. Hanes

Research output: Contribution to conferencePaper

Abstract

The development of a multi-color Confocal Particle Tracking (CPT) method for real-time correlation of intracellular gene vector transport rates with biological location in live mesenchymal stem cells was discussed. The method allows to correlate quantitative intracellular gene vector transport rates to biological locations in live cells and in real time. It was found that a small fraction of gene vectors were seen entering late endosomes/lysosome (LE/Lys) in real time, possibly coming from EE vesicles. The results show that a majority of gene vectors in EE or LE/Lys displayed anomalous sub-diffusive transport, resulting in minimal displacements from their initial position.

Original languageEnglish (US)
Pages2187-2188
Number of pages2
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

ASJC Scopus subject areas

  • Engineering(all)

Fingerprint Dive into the research topics of 'Real-time correlation of intracellular gene vector transport rates with biological location in live mesenchymal stem cells'. Together they form a unique fingerprint.

  • Cite this

    Suh, J., An, Y., Tang, B., Suk, J. S., & Hanes, J. (2004). Real-time correlation of intracellular gene vector transport rates with biological location in live mesenchymal stem cells. 2187-2188. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.