Real-time calculation and visualization of spectra in field-cycled dynamic nuclear polarization spectroscopy

Yuanmu Deng, Keerthi Shet, Haihong Li, Periannan Kuppusamy, Jay L. Zweier

Research output: Contribution to journalArticlepeer-review

Abstract

Field-cycled dynamic nuclear polarization (FC-DNP), which is based on the Overhauser effect, provides a new way to perform in vivo measurements of free radicals in biological systems. Since it measures the alterations of the nuclear magnetic resonance (NMR) signal in the presence of paramagnetic molecules, a customized program is usually needed in FC-DNP experiments to extract spectral information from the acquired NMR signals. While this program can be designed to calculate the spectrum after all the NMR signals are collected, the batch-processing mode inevitably causes delay and is not convenient for in vivo applications. In this paper, we report the development of a real-time DNP spectrum calculation and visualization program, called RT_DNP, for FC-DNP experiments. A dynamic data exchange (DDE) client was implemented to enable real-time receipt of the system information and the NMR signals from a commercial NMR console. The received NMR signals and experimental parameters were then used to calculate the DNP spectrum during the data acquisition. The real-time DNP spectrum calculation and visualization program was tested in experiments. A seamless integration of the program into a commercial NMR console has been achieved.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalComputer Methods and Programs in Biomedicine
Volume82
Issue number1
DOIs
StatePublished - Apr 2006
Externally publishedYes

Keywords

  • DNP
  • Dynamic nuclear polarization
  • EPRI
  • FC-DNP
  • MRI
  • Overhauser effect

ASJC Scopus subject areas

  • Software

Fingerprint Dive into the research topics of 'Real-time calculation and visualization of spectra in field-cycled dynamic nuclear polarization spectroscopy'. Together they form a unique fingerprint.

Cite this