Reaginic antibody formation in the mouse. III. Collaboration between hapten specific memory cells and carrier specific helper cells for secondary anti hapten antibody formation

Priming immunization of DBA/1 strain of mice with dinitrophenylated Ascaris antigen (DNP Asc) in alum and supplemental immunization with ovalbumin (OA) prepared the animals to give a typical secondary antihapten antibody response to DNP OA with respect to both IgE and IgG antibodies. The results showed collaboration between carrier specific helper cells and hapten specific memory cells for antihapten IgE antibody response. Priming with DNP Asc alone significantly enhanced antiDNP IgE and IgG antibody response to DNP OA. Enhancement of the antibody response to the DNP heterologous carrier was due to proliferation of DNP specific memory cells by priming with DNP Asc. Evidence was obtained that the DNP specific memory cells collaborated with helper cells specific for secondary carrier to show the enhanced antihapten antibody response.