Read-through activation of transcription in a cellular genomic context

Li Shen, David J. Spector

Research output: Contribution to journalArticle

Abstract

Read-through transcription from the adjacent E1a gene region is required for wild-type (wt) activity of the downstream adenovirus E1b promoter early after infection (read-through activation). However, whether a cellular chromosomal template can support read-through activation is not known. To address this issue, read-through activation was evaluated in the context of stably expressed templates in transfected cells. Inhibition of read-through transcription by insertion of a transcription termination sequence between the E1a and E1b promoters reduced downstream gene expression from stably integrated templates. The results indicate that the mechanism of read-through activation does not depend on the structure of early adenovirus nucleoprotein complexes, a structure that is likely to be different from that of cellular chromatin. Accordingly, this regulatory interaction could participate in the coordinated control of the expression of closely linked cellular genes.

Original languageEnglish (US)
Article numbere15704
JournalPLoS One
Volume5
Issue number12
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

transcriptional activation
Transcription
Adenoviridae
Transcriptional Activation
transcription (genetics)
Chemical activation
genomics
Nucleoproteins
promoter regions
Genes
Chromatin
nucleoproteins
Gene Expression
chromatin
genes
Infection
Gene expression
gene expression
infection
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Read-through activation of transcription in a cellular genomic context. / Shen, Li; Spector, David J.

In: PLoS One, Vol. 5, No. 12, e15704, 2010.

Research output: Contribution to journalArticle

Shen, Li ; Spector, David J. / Read-through activation of transcription in a cellular genomic context. In: PLoS One. 2010 ; Vol. 5, No. 12.
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